A precursor of cytoplasmic actin in cultured Drosophila cells

In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1979
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110386/
https://www.ncbi.nlm.nih.gov/pubmed/110818
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collection PubMed
description In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
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spelling pubmed-21103862008-05-01 A precursor of cytoplasmic actin in cultured Drosophila cells J Cell Biol Articles In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min. The Rockefeller University Press 1979-06-01 /pmc/articles/PMC2110386/ /pubmed/110818 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A precursor of cytoplasmic actin in cultured Drosophila cells
title A precursor of cytoplasmic actin in cultured Drosophila cells
title_full A precursor of cytoplasmic actin in cultured Drosophila cells
title_fullStr A precursor of cytoplasmic actin in cultured Drosophila cells
title_full_unstemmed A precursor of cytoplasmic actin in cultured Drosophila cells
title_short A precursor of cytoplasmic actin in cultured Drosophila cells
title_sort precursor of cytoplasmic actin in cultured drosophila cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110386/
https://www.ncbi.nlm.nih.gov/pubmed/110818

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