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Expression of microtubule networks in normal cells, transformed cells, and their hybrids

Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin re...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1979
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110424/
https://www.ncbi.nlm.nih.gov/pubmed/383725
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description Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin reacts well with mammalian tubulin. We have examined normal and transformed cells of mouse and human origin for microtubules by indirect immunofluorescence methods. Extensive networks of microtubules (MN) are easily detectable in normal and some transformed cells. The fixation procedure employed and the morphology and the cellular attachment properties seem to determine the ease of detection of MN in these cells. Cells derived from tumors and exhibiting several transformed phenotypes contained MN comparable to those of normal cells. Hybrids between transformed mouse cells and normal human cells were examined. They showed a variability in morphology, but all contained MN. These hybrids exhibited several transformed phenotypes. We conclude that in the cell lines we have examined there is no correlation between the transformed phenotypes and the organization of tubulin.
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spelling pubmed-21104242008-05-01 Expression of microtubule networks in normal cells, transformed cells, and their hybrids J Cell Biol Articles Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin reacts well with mammalian tubulin. We have examined normal and transformed cells of mouse and human origin for microtubules by indirect immunofluorescence methods. Extensive networks of microtubules (MN) are easily detectable in normal and some transformed cells. The fixation procedure employed and the morphology and the cellular attachment properties seem to determine the ease of detection of MN in these cells. Cells derived from tumors and exhibiting several transformed phenotypes contained MN comparable to those of normal cells. Hybrids between transformed mouse cells and normal human cells were examined. They showed a variability in morphology, but all contained MN. These hybrids exhibited several transformed phenotypes. We conclude that in the cell lines we have examined there is no correlation between the transformed phenotypes and the organization of tubulin. The Rockefeller University Press 1979-07-01 /pmc/articles/PMC2110424/ /pubmed/383725 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title_full Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title_fullStr Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title_full_unstemmed Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title_short Expression of microtubule networks in normal cells, transformed cells, and their hybrids
title_sort expression of microtubule networks in normal cells, transformed cells, and their hybrids
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110424/
https://www.ncbi.nlm.nih.gov/pubmed/383725