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Expression of microtubule networks in normal cells, transformed cells, and their hybrids
Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin re...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1979
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110424/ https://www.ncbi.nlm.nih.gov/pubmed/383725 |
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collection | PubMed |
description | Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin reacts well with mammalian tubulin. We have examined normal and transformed cells of mouse and human origin for microtubules by indirect immunofluorescence methods. Extensive networks of microtubules (MN) are easily detectable in normal and some transformed cells. The fixation procedure employed and the morphology and the cellular attachment properties seem to determine the ease of detection of MN in these cells. Cells derived from tumors and exhibiting several transformed phenotypes contained MN comparable to those of normal cells. Hybrids between transformed mouse cells and normal human cells were examined. They showed a variability in morphology, but all contained MN. These hybrids exhibited several transformed phenotypes. We conclude that in the cell lines we have examined there is no correlation between the transformed phenotypes and the organization of tubulin. |
format | Text |
id | pubmed-2110424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1979 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21104242008-05-01 Expression of microtubule networks in normal cells, transformed cells, and their hybrids J Cell Biol Articles Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin reacts well with mammalian tubulin. We have examined normal and transformed cells of mouse and human origin for microtubules by indirect immunofluorescence methods. Extensive networks of microtubules (MN) are easily detectable in normal and some transformed cells. The fixation procedure employed and the morphology and the cellular attachment properties seem to determine the ease of detection of MN in these cells. Cells derived from tumors and exhibiting several transformed phenotypes contained MN comparable to those of normal cells. Hybrids between transformed mouse cells and normal human cells were examined. They showed a variability in morphology, but all contained MN. These hybrids exhibited several transformed phenotypes. We conclude that in the cell lines we have examined there is no correlation between the transformed phenotypes and the organization of tubulin. The Rockefeller University Press 1979-07-01 /pmc/articles/PMC2110424/ /pubmed/383725 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title | Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title_full | Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title_fullStr | Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title_full_unstemmed | Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title_short | Expression of microtubule networks in normal cells, transformed cells, and their hybrids |
title_sort | expression of microtubule networks in normal cells, transformed cells, and their hybrids |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110424/ https://www.ncbi.nlm.nih.gov/pubmed/383725 |