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High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements
Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different cl...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1979
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110435/ https://www.ncbi.nlm.nih.gov/pubmed/574514 |
| _version_ | 1782139575096836096 |
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| collection | PubMed |
| description | Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells. |
| format | Text |
| id | pubmed-2110435 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1979 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21104352008-05-01 High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements J Cell Biol Articles Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells. The Rockefeller University Press 1979-10-01 /pmc/articles/PMC2110435/ /pubmed/574514 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title | High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title_full | High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title_fullStr | High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title_full_unstemmed | High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title_short | High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| title_sort | high resolution scanning electron microscopy of isolated and in situ cytoskeletal elements |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110435/ https://www.ncbi.nlm.nih.gov/pubmed/574514 |