Cargando…
Microtubule initiation at kinetochores and centrosomes in lysed mitotic cells. Inhibition of site-specific nucleation by tubulin antibody
A lysed cell system was developed to determine whether tubulin antibody can block the nucleation of exogenous tubulin at kinetochores and centrosomes. Mitotic PtK2 cells were pretreated with colcemid to remove all endogenous microtubules and were lysed with Triton X-100 in PIPES- EGTA-Mg++ buffer. T...
| Formato: | Texto |
|---|---|
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1979
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110471/ https://www.ncbi.nlm.nih.gov/pubmed/479317 |