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Synthesis of rat liver microsomal cytochrome b5 by free ribosomes
Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cyt...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110564/ https://www.ncbi.nlm.nih.gov/pubmed/7358795 |
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collection | PubMed |
description | Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cytosol proteins. In contrast, translation products of poly A+RNA from membrane-bound polyribosomes (poly A+RNAmb) showed some similarity to rat serum. Antibody to purified rat serum albumin immunoprecipitated from only the translation products of poly A+RNAmb a single polypeptide of mol wt 68,000. i.e., 3,000 greater than secreted serum albumin. In contrast, antibody to detergent-extracted cytochrome b5 immunoprecipitated from only the translation products of poly A+RNAf a single polypeptide of mol wt 17,500, identical to that of microsomal cytochrome b5. A consideration of the known properties of cytochrome b5 is consistent with an exclusive site of synthesis on free ribosomes. |
format | Text |
id | pubmed-2110564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21105642008-05-01 Synthesis of rat liver microsomal cytochrome b5 by free ribosomes J Cell Biol Articles Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cytosol proteins. In contrast, translation products of poly A+RNA from membrane-bound polyribosomes (poly A+RNAmb) showed some similarity to rat serum. Antibody to purified rat serum albumin immunoprecipitated from only the translation products of poly A+RNAmb a single polypeptide of mol wt 68,000. i.e., 3,000 greater than secreted serum albumin. In contrast, antibody to detergent-extracted cytochrome b5 immunoprecipitated from only the translation products of poly A+RNAf a single polypeptide of mol wt 17,500, identical to that of microsomal cytochrome b5. A consideration of the known properties of cytochrome b5 is consistent with an exclusive site of synthesis on free ribosomes. The Rockefeller University Press 1980-03-01 /pmc/articles/PMC2110564/ /pubmed/7358795 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title | Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title_full | Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title_fullStr | Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title_full_unstemmed | Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title_short | Synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
title_sort | synthesis of rat liver microsomal cytochrome b5 by free ribosomes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110564/ https://www.ncbi.nlm.nih.gov/pubmed/7358795 |