Cargando…

Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells

Our previous studies showed that 10 percent dimethyl sulfoxide (DMSO) induces the formation of actin microfilament bundles in the cell nucleus together with the dislocation of cortical microfilaments from the plasma membrane. The present study investigated the effects of DMSO on diverse activities m...

Descripción completa

Detalles Bibliográficos
Autor principal: Fukui, Y
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1980
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110636/
https://www.ncbi.nlm.nih.gov/pubmed/6893450
_version_ 1782139629613350912
author Fukui, Y
author_facet Fukui, Y
author_sort Fukui, Y
collection PubMed
description Our previous studies showed that 10 percent dimethyl sulfoxide (DMSO) induces the formation of actin microfilament bundles in the cell nucleus together with the dislocation of cortical microfilaments from the plasma membrane. The present study investigated the effects of DMSO on diverse activities mediated by cellular microfilaments as the second step toward assessing potential differences between nuclear and cytoplasmic actins of dictyostelium mucoroides. DMSO was found to reversibly inhibit cell-to- glass as well as cell-to-cell adhesion, cell locomotion, and cell multiplication, whereas cytoplasmic streaming and phagocytosis were not obviously inhibited. Also, 5 percent DMSO inhibited cytokinesis but did not totally inhibit cell growth thus leading to the development of giant cells more than 10 times larger than normal cells. Transmission electron microscopy using serial thin sections showed the occurrence of multinucleation in the DMSO- induced giant cells. After the removal of DMSO, the giant multinuclear cells underwent multiple cytoplasmic cleavage producing normal-sized mononuclear cells. The nuclear division in the DMSO-induced giant cells was unique in that no spindle microtubules were formed, and vesicles appeared inside the nucleus forming a transverse partition of the nuclear envelope. The presence of actin filaments in those nuclei was demonstrated by a binding study with skeletal muscle myosin subfragment-1, and their possible involvement in this mode of nuclear division is discussed.
format Text
id pubmed-2110636
institution National Center for Biotechnology Information
language English
publishDate 1980
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21106362008-05-01 Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells Fukui, Y J Cell Biol Articles Our previous studies showed that 10 percent dimethyl sulfoxide (DMSO) induces the formation of actin microfilament bundles in the cell nucleus together with the dislocation of cortical microfilaments from the plasma membrane. The present study investigated the effects of DMSO on diverse activities mediated by cellular microfilaments as the second step toward assessing potential differences between nuclear and cytoplasmic actins of dictyostelium mucoroides. DMSO was found to reversibly inhibit cell-to- glass as well as cell-to-cell adhesion, cell locomotion, and cell multiplication, whereas cytoplasmic streaming and phagocytosis were not obviously inhibited. Also, 5 percent DMSO inhibited cytokinesis but did not totally inhibit cell growth thus leading to the development of giant cells more than 10 times larger than normal cells. Transmission electron microscopy using serial thin sections showed the occurrence of multinucleation in the DMSO- induced giant cells. After the removal of DMSO, the giant multinuclear cells underwent multiple cytoplasmic cleavage producing normal-sized mononuclear cells. The nuclear division in the DMSO-induced giant cells was unique in that no spindle microtubules were formed, and vesicles appeared inside the nucleus forming a transverse partition of the nuclear envelope. The presence of actin filaments in those nuclei was demonstrated by a binding study with skeletal muscle myosin subfragment-1, and their possible involvement in this mode of nuclear division is discussed. The Rockefeller University Press 1980-07-01 /pmc/articles/PMC2110636/ /pubmed/6893450 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Fukui, Y
Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title_full Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title_fullStr Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title_full_unstemmed Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title_short Formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
title_sort formation of multinuclear cells induced by dimethyl sulfoxide: inhibition of cytokinesis and occurrence of novel nuclear division in dictyostelium cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110636/
https://www.ncbi.nlm.nih.gov/pubmed/6893450
work_keys_str_mv AT fukuiy formationofmultinuclearcellsinducedbydimethylsulfoxideinhibitionofcytokinesisandoccurrenceofnovelnucleardivisionindictyosteliumcells