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An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins
We elicited antibodies in rabbits to actin purified from body wall muscle of the marine mollusc, Aplysia californica. We found that this antiactin has an unusual specificity: in addition to reacting with the immunogen, it recognizes cytoplasmic vertebrate actins but not myofibrillar actin. Radioimmu...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1980
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110679/ https://www.ncbi.nlm.nih.gov/pubmed/6893333 |
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collection | PubMed |
description | We elicited antibodies in rabbits to actin purified from body wall muscle of the marine mollusc, Aplysia californica. We found that this antiactin has an unusual specificity: in addition to reacting with the immunogen, it recognizes cytoplasmic vertebrate actins but not myofibrillar actin. Radioimmunoassay showed little or no cross-reaction with actin purified from either chicken gizzard or rabbit skeletal muscle. Immunocytochemical studies with human fibroblasts and L6 myoblasts revealed intense staining of typical cytoplasmic cables. Myofibrils were not stained after treatment of human and frog skeletal muscle with the antibody, although the distribution of immunofluorescence suggested that cytoplasmic actin is associated with membrane systems in the muscle fiber. The antibody may therefore be especially suited for studying the localization of cytoplasmic actin in skeletal muscle cells even in the presence of a great excess of the myofibrillar form. |
format | Text |
id | pubmed-2110679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1980 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21106792008-05-01 An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins J Cell Biol Articles We elicited antibodies in rabbits to actin purified from body wall muscle of the marine mollusc, Aplysia californica. We found that this antiactin has an unusual specificity: in addition to reacting with the immunogen, it recognizes cytoplasmic vertebrate actins but not myofibrillar actin. Radioimmunoassay showed little or no cross-reaction with actin purified from either chicken gizzard or rabbit skeletal muscle. Immunocytochemical studies with human fibroblasts and L6 myoblasts revealed intense staining of typical cytoplasmic cables. Myofibrils were not stained after treatment of human and frog skeletal muscle with the antibody, although the distribution of immunofluorescence suggested that cytoplasmic actin is associated with membrane systems in the muscle fiber. The antibody may therefore be especially suited for studying the localization of cytoplasmic actin in skeletal muscle cells even in the presence of a great excess of the myofibrillar form. The Rockefeller University Press 1980-09-01 /pmc/articles/PMC2110679/ /pubmed/6893333 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title | An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title_full | An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title_fullStr | An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title_full_unstemmed | An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title_short | An antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
title_sort | antiactin antibody that distinguishes between cytoplasmic and skeletal muscle actins |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110679/ https://www.ncbi.nlm.nih.gov/pubmed/6893333 |