Cargando…

Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes

A new procedure is introduced for the isolation of connective tissue fibers, called biomatrix, containing a significant portion of the extracellular matrix (basement membrane components and components of the ground substance). Biomatrix isolated from normal rat liver contains >90% of the tissue&#...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1980
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110711/
https://www.ncbi.nlm.nih.gov/pubmed/7419593
_version_ 1782139648639762432
collection PubMed
description A new procedure is introduced for the isolation of connective tissue fibers, called biomatrix, containing a significant portion of the extracellular matrix (basement membrane components and components of the ground substance). Biomatrix isolated from normal rat liver contains >90% of the tissue's collagens and all of the known collagen types, including types I and III and basement membrane collagens. The purified collagenous fibers are associated with noncollagenous acidic proteins (including fibronectins and possibly small amounts of glycosaminoglycans). Procedures are also described for preparing tissue culture substrates with these fibers by either smearing tissue culture dishes with frozen sections or by shredding the biomatrix into small fibrils with a homogenizer. The biomatrix as a substrate has a remarkable ability to sustain normal rat hepatocytes long-term in culture. The hepatocytes, which on tissue culture plastic or on type I collagen gels do not survive more than a few weeks, have been maintained for more than 5 mo in vitro when cultured on biomatrix. These cells cultured on rat liver biomatrix show increased attachment and survival efficiencies, long-term survival (months) and retention of some hepatocyte-specific functions.
format Text
id pubmed-2110711
institution National Center for Biotechnology Information
language English
publishDate 1980
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21107112008-05-01 Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes J Cell Biol Articles A new procedure is introduced for the isolation of connective tissue fibers, called biomatrix, containing a significant portion of the extracellular matrix (basement membrane components and components of the ground substance). Biomatrix isolated from normal rat liver contains >90% of the tissue's collagens and all of the known collagen types, including types I and III and basement membrane collagens. The purified collagenous fibers are associated with noncollagenous acidic proteins (including fibronectins and possibly small amounts of glycosaminoglycans). Procedures are also described for preparing tissue culture substrates with these fibers by either smearing tissue culture dishes with frozen sections or by shredding the biomatrix into small fibrils with a homogenizer. The biomatrix as a substrate has a remarkable ability to sustain normal rat hepatocytes long-term in culture. The hepatocytes, which on tissue culture plastic or on type I collagen gels do not survive more than a few weeks, have been maintained for more than 5 mo in vitro when cultured on biomatrix. These cells cultured on rat liver biomatrix show increased attachment and survival efficiencies, long-term survival (months) and retention of some hepatocyte-specific functions. The Rockefeller University Press 1980-10-01 /pmc/articles/PMC2110711/ /pubmed/7419593 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title_full Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title_fullStr Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title_full_unstemmed Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title_short Connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
title_sort connective tissue biomatrix: its isolation and utilization for long- term cultures of normal rat hepatocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110711/
https://www.ncbi.nlm.nih.gov/pubmed/7419593