Electron microscope evidence for the presence of globular structures in different sperm chromatins

Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron mi...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1980
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110730/
https://www.ncbi.nlm.nih.gov/pubmed/7419596
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collection PubMed
description Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron microscopy of such dispersed nuclear fibers has shown a beads-on-a-string configuration for these four types of sperm chromatin. These structures are morphologically similar to those described in somatic cell nuclei as nucleosomes, although in sperm chromatin the basic proteins associated with DNA were significantly different from histones.
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spelling pubmed-21107302008-05-01 Electron microscope evidence for the presence of globular structures in different sperm chromatins J Cell Biol Articles Dispersion of nuclear fibers of the spermatozoa of dogfish, man, and bull is made possible after treatment with a reducing and alkylating reagent coupled with an anionic detergent; the same detergent used at a low ionic strength dissociates the nuclear content of the rainbow trout sperm. Electron microscopy of such dispersed nuclear fibers has shown a beads-on-a-string configuration for these four types of sperm chromatin. These structures are morphologically similar to those described in somatic cell nuclei as nucleosomes, although in sperm chromatin the basic proteins associated with DNA were significantly different from histones. The Rockefeller University Press 1980-10-01 /pmc/articles/PMC2110730/ /pubmed/7419596 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Electron microscope evidence for the presence of globular structures in different sperm chromatins
title Electron microscope evidence for the presence of globular structures in different sperm chromatins
title_full Electron microscope evidence for the presence of globular structures in different sperm chromatins
title_fullStr Electron microscope evidence for the presence of globular structures in different sperm chromatins
title_full_unstemmed Electron microscope evidence for the presence of globular structures in different sperm chromatins
title_short Electron microscope evidence for the presence of globular structures in different sperm chromatins
title_sort electron microscope evidence for the presence of globular structures in different sperm chromatins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110730/
https://www.ncbi.nlm.nih.gov/pubmed/7419596

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