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Visualization of nucleosomes in thin sections by stereo electron microscopy
Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supr...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1980
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110767/ https://www.ncbi.nlm.nih.gov/pubmed/7007397 |
| _version_ | 1782139662951776256 |
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| collection | PubMed |
| description | Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stability of nucleosome structure in the dispersed chromatin state during fixation, dehydration, and embedding. |
| format | Text |
| id | pubmed-2110767 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1980 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21107672008-05-01 Visualization of nucleosomes in thin sections by stereo electron microscopy J Cell Biol Articles Nucleosomes (approximately diameter) were clearly visualized in thin sections (approximately 0.1 micrometer thick) of isolated chicken erythrocytes. The cells were lysed and fixed in low ionic strength buffers that maintained the chromatin as dispersed filaments and prevented the reformation of supranucleosomal structures. Stereo electron micrographs at high magnification demonstrate the stability of nucleosome structure in the dispersed chromatin state during fixation, dehydration, and embedding. The Rockefeller University Press 1980-12-01 /pmc/articles/PMC2110767/ /pubmed/7007397 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title | Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title_full | Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title_fullStr | Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title_full_unstemmed | Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title_short | Visualization of nucleosomes in thin sections by stereo electron microscopy |
| title_sort | visualization of nucleosomes in thin sections by stereo electron microscopy |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110767/ https://www.ncbi.nlm.nih.gov/pubmed/7007397 |