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PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)

The degradation of cellular proteins in fibroblasts, both those of rapid and those of slow turnover rates, was inhibited by low concentrations of chloroquine or neutral red in the medium. Cells inhibited by chloroquine can be inhibited further by fluoride. Chloroquine was taken up by the fibroblasts...

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Detalles Bibliográficos
Autores principales: Wibo, Maurice, Poole, Brian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110942/
https://www.ncbi.nlm.nih.gov/pubmed/4607946
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author Wibo, Maurice
Poole, Brian
author_facet Wibo, Maurice
Poole, Brian
author_sort Wibo, Maurice
collection PubMed
description The degradation of cellular proteins in fibroblasts, both those of rapid and those of slow turnover rates, was inhibited by low concentrations of chloroquine or neutral red in the medium. Cells inhibited by chloroquine can be inhibited further by fluoride. Chloroquine was taken up by the fibroblasts and the concentration in the cells reached several hundred times that in the medium. Isopycnic fractionation studies showed that within the cells the chloroquine was concentrated in the lysosomes, and that these chloroquine-containing lysosomes had a lower equilibrium density than the lysosomes of untreated cells. Chloroquine, at concentrations attained inside the lysosomes, inhibited cathepsin B(1) but not cathepsin D. It is concluded that chloroquine impairs the breakdown of cellular proteins after these have entered the lysosome system, probably through inhibition of cathepsin B(1).
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spelling pubmed-21109422008-05-01 PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1) Wibo, Maurice Poole, Brian J Cell Biol Article The degradation of cellular proteins in fibroblasts, both those of rapid and those of slow turnover rates, was inhibited by low concentrations of chloroquine or neutral red in the medium. Cells inhibited by chloroquine can be inhibited further by fluoride. Chloroquine was taken up by the fibroblasts and the concentration in the cells reached several hundred times that in the medium. Isopycnic fractionation studies showed that within the cells the chloroquine was concentrated in the lysosomes, and that these chloroquine-containing lysosomes had a lower equilibrium density than the lysosomes of untreated cells. Chloroquine, at concentrations attained inside the lysosomes, inhibited cathepsin B(1) but not cathepsin D. It is concluded that chloroquine impairs the breakdown of cellular proteins after these have entered the lysosome system, probably through inhibition of cathepsin B(1). The Rockefeller University Press 1974-11-01 /pmc/articles/PMC2110942/ /pubmed/4607946 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wibo, Maurice
Poole, Brian
PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title_full PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title_fullStr PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title_full_unstemmed PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title_short PROTEIN DEGRADATION IN CULTURED CELLS : II. The Uptake of Chloroquine by Rat Fibroblasts and the Inhibition of Cellular Protein Degradation and Cathepsin B(1)
title_sort protein degradation in cultured cells : ii. the uptake of chloroquine by rat fibroblasts and the inhibition of cellular protein degradation and cathepsin b(1)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110942/
https://www.ncbi.nlm.nih.gov/pubmed/4607946
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