AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS

We describe a vibrating probe system for measuring relatively steady electrical current densities near individual living cells. It has a signal-to-noise ratio at least 100 times greater than previously available techniques. Thus it can be used to detect current densities as small as 10 nA/cm(2) in s...

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Detalles Bibliográficos
Autores principales: Jaffe, Lionel F., Nuccitelli, Richard
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110946/
https://www.ncbi.nlm.nih.gov/pubmed/4421919
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author Jaffe, Lionel F.
Nuccitelli, Richard
author_facet Jaffe, Lionel F.
Nuccitelli, Richard
author_sort Jaffe, Lionel F.
collection PubMed
description We describe a vibrating probe system for measuring relatively steady electrical current densities near individual living cells. It has a signal-to-noise ratio at least 100 times greater than previously available techniques. Thus it can be used to detect current densities as small as 10 nA/cm(2) in serum when a 30-µm diameter probe is vibrated at 200 Hz between two points 30 µm apart, and the amplifier's time constant is set at 10 s. Moreover, it should be generally insensitive to interference by concentration gradients. It has been first used to reveal and study 100-s long current pulses which developing fucoid embryos drive through themselves.
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spelling pubmed-21109462008-05-01 AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS Jaffe, Lionel F. Nuccitelli, Richard J Cell Biol Article We describe a vibrating probe system for measuring relatively steady electrical current densities near individual living cells. It has a signal-to-noise ratio at least 100 times greater than previously available techniques. Thus it can be used to detect current densities as small as 10 nA/cm(2) in serum when a 30-µm diameter probe is vibrated at 200 Hz between two points 30 µm apart, and the amplifier's time constant is set at 10 s. Moreover, it should be generally insensitive to interference by concentration gradients. It has been first used to reveal and study 100-s long current pulses which developing fucoid embryos drive through themselves. The Rockefeller University Press 1974-11-01 /pmc/articles/PMC2110946/ /pubmed/4421919 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Jaffe, Lionel F.
Nuccitelli, Richard
AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title_full AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title_fullStr AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title_full_unstemmed AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title_short AN ULTRASENSITIVE VIBRATING PROBE FOR MEASURING STEADY EXTRACELLULAR CURRENTS
title_sort ultrasensitive vibrating probe for measuring steady extracellular currents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110946/
https://www.ncbi.nlm.nih.gov/pubmed/4421919
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