HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes

A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. I...

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Detalles Bibliográficos
Autores principales: Johnsen, Sven, Stokke, Torbjørn, Prydz, Hans
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110951/
https://www.ncbi.nlm.nih.gov/pubmed/4278376
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author Johnsen, Sven
Stokke, Torbjørn
Prydz, Hans
author_facet Johnsen, Sven
Stokke, Torbjørn
Prydz, Hans
author_sort Johnsen, Sven
collection PubMed
description A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fractions differed from that of ouabain-sensitive ATPase. In addition, the specific activity of 5'-nucleotidase did not follow the distribution of the membrane ghosts. Thus, this enzyme would seem unsuitable as a plasma membrane marker. A complete balance sheet for marker enzyme activities during the fractionation is necessary for the calculation of increase in specific activity because the activities of both 5'-nucleotidase and ouabain-sensitive ATPase might change during the fractionation procedures.
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spelling pubmed-21109512008-05-01 HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes Johnsen, Sven Stokke, Torbjørn Prydz, Hans J Cell Biol Article A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fractions differed from that of ouabain-sensitive ATPase. In addition, the specific activity of 5'-nucleotidase did not follow the distribution of the membrane ghosts. Thus, this enzyme would seem unsuitable as a plasma membrane marker. A complete balance sheet for marker enzyme activities during the fractionation is necessary for the calculation of increase in specific activity because the activities of both 5'-nucleotidase and ouabain-sensitive ATPase might change during the fractionation procedures. The Rockefeller University Press 1974-11-01 /pmc/articles/PMC2110951/ /pubmed/4278376 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Johnsen, Sven
Stokke, Torbjørn
Prydz, Hans
HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title_full HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title_fullStr HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title_full_unstemmed HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title_short HELA CELL PLASMA MEMBRANES : I. 5'-Nucleotidase and Ouabain-Sensitive ATPase as Markers for Plasma Membranes
title_sort hela cell plasma membranes : i. 5'-nucleotidase and ouabain-sensitive atpase as markers for plasma membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110951/
https://www.ncbi.nlm.nih.gov/pubmed/4278376
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AT prydzhans helacellplasmamembranesi5nucleotidaseandouabainsensitiveatpaseasmarkersforplasmamembranes

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