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Freeze-fracture of monolayer cultures
This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both ti...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1977
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111038/ https://www.ncbi.nlm.nih.gov/pubmed/838774 |
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collection | PubMed |
description | This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing. |
format | Text |
id | pubmed-2111038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21110382008-05-01 Freeze-fracture of monolayer cultures J Cell Biol Articles This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing. The Rockefeller University Press 1977-03-01 /pmc/articles/PMC2111038/ /pubmed/838774 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Freeze-fracture of monolayer cultures |
title | Freeze-fracture of monolayer cultures |
title_full | Freeze-fracture of monolayer cultures |
title_fullStr | Freeze-fracture of monolayer cultures |
title_full_unstemmed | Freeze-fracture of monolayer cultures |
title_short | Freeze-fracture of monolayer cultures |
title_sort | freeze-fracture of monolayer cultures |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111038/ https://www.ncbi.nlm.nih.gov/pubmed/838774 |