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Freeze-fracture of monolayer cultures

This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both ti...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111038/
https://www.ncbi.nlm.nih.gov/pubmed/838774
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description This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.
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spelling pubmed-21110382008-05-01 Freeze-fracture of monolayer cultures J Cell Biol Articles This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing. The Rockefeller University Press 1977-03-01 /pmc/articles/PMC2111038/ /pubmed/838774 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Freeze-fracture of monolayer cultures
title Freeze-fracture of monolayer cultures
title_full Freeze-fracture of monolayer cultures
title_fullStr Freeze-fracture of monolayer cultures
title_full_unstemmed Freeze-fracture of monolayer cultures
title_short Freeze-fracture of monolayer cultures
title_sort freeze-fracture of monolayer cultures
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111038/
https://www.ncbi.nlm.nih.gov/pubmed/838774