Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy

A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111432/
https://www.ncbi.nlm.nih.gov/pubmed/326797
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collection PubMed
description A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.
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spelling pubmed-21114322008-05-01 Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy J Cell Biol Articles A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin. The Rockefeller University Press 1977-06-01 /pmc/articles/PMC2111432/ /pubmed/326797 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title_full Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title_fullStr Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title_full_unstemmed Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title_short Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
title_sort use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111432/
https://www.ncbi.nlm.nih.gov/pubmed/326797

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