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Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling
Glial fibrillary acidic protein was localized at the electron microscope level in the cerebellum of adult mice by indirect immunoperoxidase histology. In confirmation of previous studies at the light microscope level, the antigen was detectable in astrocytes and their processes, but not in neurons o...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111556/ https://www.ncbi.nlm.nih.gov/pubmed/334780 |
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collection | PubMed |
description | Glial fibrillary acidic protein was localized at the electron microscope level in the cerebellum of adult mice by indirect immunoperoxidase histology. In confirmation of previous studies at the light microscope level, the antigen was detectable in astrocytes and their processes, but not in neurons or their processes, or in oligodendroglia. Astrocytic processes were stained in white matter, in the granular layet surrounding synaptic glomerular complexes, and in the molecular layer in the form of radially oriented fibers and of sheaths surrounding Purkinje cell dendrites. Astrocytic endfeet impinging on meninges and perivascular membranes were also antigen positive. In astrocytic perikarya and processes, the immunohistochemical reaction product appears both as a diffuse cytoplasmic label and as elongated strands, which by their distribution and frequency could be considered glial filaments. |
format | Text |
id | pubmed-2111556 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21115562008-05-01 Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling J Cell Biol Articles Glial fibrillary acidic protein was localized at the electron microscope level in the cerebellum of adult mice by indirect immunoperoxidase histology. In confirmation of previous studies at the light microscope level, the antigen was detectable in astrocytes and their processes, but not in neurons or their processes, or in oligodendroglia. Astrocytic processes were stained in white matter, in the granular layet surrounding synaptic glomerular complexes, and in the molecular layer in the form of radially oriented fibers and of sheaths surrounding Purkinje cell dendrites. Astrocytic endfeet impinging on meninges and perivascular membranes were also antigen positive. In astrocytic perikarya and processes, the immunohistochemical reaction product appears both as a diffuse cytoplasmic label and as elongated strands, which by their distribution and frequency could be considered glial filaments. The Rockefeller University Press 1977-10-01 /pmc/articles/PMC2111556/ /pubmed/334780 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title | Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title_full | Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title_fullStr | Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title_full_unstemmed | Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title_short | Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
title_sort | ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111556/ https://www.ncbi.nlm.nih.gov/pubmed/334780 |