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Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum
Coated vesicles from the brain have been purified to near morphological homogeneity by a modification of the method of Pearse. These vesicles resemble sarcoplasmic reticulum fragments isolated from skeletal muscle. They contain proteins with 100,000- and 55,000-dalton mol wt which co-migrate on poly...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111570/ https://www.ncbi.nlm.nih.gov/pubmed/144139 |
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collection | PubMed |
description | Coated vesicles from the brain have been purified to near morphological homogeneity by a modification of the method of Pearse. These vesicles resemble sarcoplasmic reticulum fragments isolated from skeletal muscle. They contain proteins with 100,000- and 55,000-dalton mol wt which co-migrate on polyacrylamide gels, in the presence of sodium dodecyl sulfate, with the two major proteins of the sarcoplasmic reticulum fragment. These vesicles contain adenosine triphosphatase (ATPase) activity which is stimulated by calcium ions in the presence of Triton X-100 (Rohm & Haas Co., Philadelphia, Pa.), displaying maximal activity at 8 x 10(-7) M Ca ++. They take up calcium ions from the medium, and this uptake is stimulated by ATP and by potassium oxalate, a calcium-trapping agent. The 100,000-dalton protein of the coated vesicles displays immunological reactivity with an antiserum directed against the 100,000-dalton, calcium-stimulated ATPase of the sarcoplasmic reticulum. As with the sarcoplasmic reticulum fragment, this protein becomes radiolabeled when coated vesicles are briefly incubated with gamma-labeled [32P]ATP. The possible functions of coated vesicles as calcium-sequestering organelles are discussed. |
format | Text |
id | pubmed-2111570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21115702008-05-01 Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum J Cell Biol Articles Coated vesicles from the brain have been purified to near morphological homogeneity by a modification of the method of Pearse. These vesicles resemble sarcoplasmic reticulum fragments isolated from skeletal muscle. They contain proteins with 100,000- and 55,000-dalton mol wt which co-migrate on polyacrylamide gels, in the presence of sodium dodecyl sulfate, with the two major proteins of the sarcoplasmic reticulum fragment. These vesicles contain adenosine triphosphatase (ATPase) activity which is stimulated by calcium ions in the presence of Triton X-100 (Rohm & Haas Co., Philadelphia, Pa.), displaying maximal activity at 8 x 10(-7) M Ca ++. They take up calcium ions from the medium, and this uptake is stimulated by ATP and by potassium oxalate, a calcium-trapping agent. The 100,000-dalton protein of the coated vesicles displays immunological reactivity with an antiserum directed against the 100,000-dalton, calcium-stimulated ATPase of the sarcoplasmic reticulum. As with the sarcoplasmic reticulum fragment, this protein becomes radiolabeled when coated vesicles are briefly incubated with gamma-labeled [32P]ATP. The possible functions of coated vesicles as calcium-sequestering organelles are discussed. The Rockefeller University Press 1977-10-01 /pmc/articles/PMC2111570/ /pubmed/144139 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title | Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title_full | Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title_fullStr | Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title_full_unstemmed | Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title_short | Evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
title_sort | evidence that coated vesicles isolated from brain are calcium- sequestering organelles resembling sarcoplasmic reticulum |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111570/ https://www.ncbi.nlm.nih.gov/pubmed/144139 |