Isolation of liver lysosomes by iron loading. Ultrastructural characterization

In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1975
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111670/
https://www.ncbi.nlm.nih.gov/pubmed/1202026
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collection PubMed
description In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The advantage of adding fixative to the bottom of the gradient and of using en bloc staining with uranyl acetate is also demonstrated.
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spelling pubmed-21116702008-05-01 Isolation of liver lysosomes by iron loading. Ultrastructural characterization J Cell Biol Articles In summary, the data demonstrate that, by the use of repeated injections of an iron sorbitol complex, it is possible to isolate a fraction highly enriched in hydrolytic enzymes (60 times over the homogenate) and in well preserved lysosomes emanating almost entirely from liver parenchymal cells. The advantage of adding fixative to the bottom of the gradient and of using en bloc staining with uranyl acetate is also demonstrated. The Rockefeller University Press 1975-12-01 /pmc/articles/PMC2111670/ /pubmed/1202026 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title_full Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title_fullStr Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title_full_unstemmed Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title_short Isolation of liver lysosomes by iron loading. Ultrastructural characterization
title_sort isolation of liver lysosomes by iron loading. ultrastructural characterization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111670/
https://www.ncbi.nlm.nih.gov/pubmed/1202026

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