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Detection of actin assembly by fluorescence energy transfer
Fluorescence energy transfer was used to measure the assembly and disassembly of actin filaments. Actin was labeled at cysteine 373 with an energy donor (5-iodoacetamidofluorescein) or an energy acceptor (tetramethylrhodamine iodoacetamide or eosin iodoacetamide). Donor- labeled actin and acceptor-l...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1981
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111691/ https://www.ncbi.nlm.nih.gov/pubmed/6894758 |