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Cell-substrate contacts illuminated by total internal reflection fluorescence

A technique for exciting fluorescence exclusively from regions of contact between cultured cells and the substrate is presented. The technique utilizes the evanescent wave of a totally internally reflecting laser beam to excite only those fluorescent molecules within one light wavelength or less of...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1981
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111781/
https://www.ncbi.nlm.nih.gov/pubmed/7014571
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collection PubMed
description A technique for exciting fluorescence exclusively from regions of contact between cultured cells and the substrate is presented. The technique utilizes the evanescent wave of a totally internally reflecting laser beam to excite only those fluorescent molecules within one light wavelength or less of the substrate surface. Demonstrations of this technique are given for two types of cell cultures: rat primary myotubes with acetylcholine receptors labeled by fluorescent alpha- bungarotoxin and human skin fibroblasts labeled by a fluorescent lipid probe. Total internal reflection fluorescence examination of cells appears to have promising applications, including visualization of the membrane and underlying cytoplasmic structures at cell-substrate contacts, dramatic reduction of autofluorescence from debris and thick cells, mapping of membranes topography, and visualization of reversible bound fluorescent ligands at membrane receptors.
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spelling pubmed-21117812008-05-01 Cell-substrate contacts illuminated by total internal reflection fluorescence J Cell Biol Articles A technique for exciting fluorescence exclusively from regions of contact between cultured cells and the substrate is presented. The technique utilizes the evanescent wave of a totally internally reflecting laser beam to excite only those fluorescent molecules within one light wavelength or less of the substrate surface. Demonstrations of this technique are given for two types of cell cultures: rat primary myotubes with acetylcholine receptors labeled by fluorescent alpha- bungarotoxin and human skin fibroblasts labeled by a fluorescent lipid probe. Total internal reflection fluorescence examination of cells appears to have promising applications, including visualization of the membrane and underlying cytoplasmic structures at cell-substrate contacts, dramatic reduction of autofluorescence from debris and thick cells, mapping of membranes topography, and visualization of reversible bound fluorescent ligands at membrane receptors. The Rockefeller University Press 1981-04-01 /pmc/articles/PMC2111781/ /pubmed/7014571 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Cell-substrate contacts illuminated by total internal reflection fluorescence
title Cell-substrate contacts illuminated by total internal reflection fluorescence
title_full Cell-substrate contacts illuminated by total internal reflection fluorescence
title_fullStr Cell-substrate contacts illuminated by total internal reflection fluorescence
title_full_unstemmed Cell-substrate contacts illuminated by total internal reflection fluorescence
title_short Cell-substrate contacts illuminated by total internal reflection fluorescence
title_sort cell-substrate contacts illuminated by total internal reflection fluorescence
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111781/
https://www.ncbi.nlm.nih.gov/pubmed/7014571