Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture

To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111995/
https://www.ncbi.nlm.nih.gov/pubmed/7199053
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collection PubMed
description To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.
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spelling pubmed-21119952008-05-01 Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture J Cell Biol Articles To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction. The Rockefeller University Press 1982-01-01 /pmc/articles/PMC2111995/ /pubmed/7199053 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title_full Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title_fullStr Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title_full_unstemmed Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title_short Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
title_sort interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2111995/
https://www.ncbi.nlm.nih.gov/pubmed/7199053

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