Cargando…

Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints

Measurements of lateral molecular diffusion on blebs formed on the surfaces of isolated muscle cells and myoblasts are reported. These blebbed membranes retain integral proteins but apparently separate from the detectable cytoskeleton. On blebs, acetylcholine receptors, concanavalin A receptors, and...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112013/
https://www.ncbi.nlm.nih.gov/pubmed/7199052
_version_ 1782139858082332672
collection PubMed
description Measurements of lateral molecular diffusion on blebs formed on the surfaces of isolated muscle cells and myoblasts are reported. These blebbed membranes retain integral proteins but apparently separate from the detectable cytoskeleton. On blebs, acetylcholine receptors, concanavalin A receptors, and stearoyldextran extrinsic model receptor molecules are free to diffuse with a diffusion coefficient (D) approximately 3 x 10(-9) cm2/s, which is close to the value predicted for hydrodynamic drag in the lipid membrane. In contrast, diffusion of these typical receptors in intact cell membranes is constrained to D approximately less than 10(-10) cm2/s with substantial fractions virtually nondiffusible (D less than 10(-12) cm2/s). Lipid analog diffusion is also slightly enhanced in blebs as expected of evanescent lipid protein interaction. This strong enhancement of membrane protein diffusion is attributed to release from unidentified natural constraints that is induced in some way by detachment of the bleb membrane.
format Text
id pubmed-2112013
institution National Center for Biotechnology Information
language English
publishDate 1982
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21120132008-05-01 Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints J Cell Biol Articles Measurements of lateral molecular diffusion on blebs formed on the surfaces of isolated muscle cells and myoblasts are reported. These blebbed membranes retain integral proteins but apparently separate from the detectable cytoskeleton. On blebs, acetylcholine receptors, concanavalin A receptors, and stearoyldextran extrinsic model receptor molecules are free to diffuse with a diffusion coefficient (D) approximately 3 x 10(-9) cm2/s, which is close to the value predicted for hydrodynamic drag in the lipid membrane. In contrast, diffusion of these typical receptors in intact cell membranes is constrained to D approximately less than 10(-10) cm2/s with substantial fractions virtually nondiffusible (D less than 10(-12) cm2/s). Lipid analog diffusion is also slightly enhanced in blebs as expected of evanescent lipid protein interaction. This strong enhancement of membrane protein diffusion is attributed to release from unidentified natural constraints that is induced in some way by detachment of the bleb membrane. The Rockefeller University Press 1982-01-01 /pmc/articles/PMC2112013/ /pubmed/7199052 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title_full Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title_fullStr Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title_full_unstemmed Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title_short Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
title_sort enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112013/
https://www.ncbi.nlm.nih.gov/pubmed/7199052