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Direct communication of homologous and heterologous endocrine islet cells in culture

The transfer of 6-carboxyfluorescein between islet cells in monolayer culture was observed by fluorescence microscopy, and the endocrine cells involved in this transfer were identified by immunohistochemistry and electron microscopy. The results show that carboxyfluorescein was directly exchanged be...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112014/
https://www.ncbi.nlm.nih.gov/pubmed/7035468
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collection PubMed
description The transfer of 6-carboxyfluorescein between islet cells in monolayer culture was observed by fluorescence microscopy, and the endocrine cells involved in this transfer were identified by immunohistochemistry and electron microscopy. The results show that carboxyfluorescein was directly exchanged between homologous B-cells and also between B- and A- or D-cells. Successive microinjections of the probe into different cells of the same cluster showed the existence of separate territories, each formed by 2-8 communicating cells. Intercellular communication was not observed after every dye microinjection, and communicating and noncommunicating islet cells were found to coexist within the same cluster. The data indicate that the exchange of exogenous cytoplasmic molecules occurs between different types of endocrine islet cells. However, within a single cluster, all islet cells are not metabolically coupled to one another, at a given time.
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spelling pubmed-21120142008-05-01 Direct communication of homologous and heterologous endocrine islet cells in culture J Cell Biol Articles The transfer of 6-carboxyfluorescein between islet cells in monolayer culture was observed by fluorescence microscopy, and the endocrine cells involved in this transfer were identified by immunohistochemistry and electron microscopy. The results show that carboxyfluorescein was directly exchanged between homologous B-cells and also between B- and A- or D-cells. Successive microinjections of the probe into different cells of the same cluster showed the existence of separate territories, each formed by 2-8 communicating cells. Intercellular communication was not observed after every dye microinjection, and communicating and noncommunicating islet cells were found to coexist within the same cluster. The data indicate that the exchange of exogenous cytoplasmic molecules occurs between different types of endocrine islet cells. However, within a single cluster, all islet cells are not metabolically coupled to one another, at a given time. The Rockefeller University Press 1982-01-01 /pmc/articles/PMC2112014/ /pubmed/7035468 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Direct communication of homologous and heterologous endocrine islet cells in culture
title Direct communication of homologous and heterologous endocrine islet cells in culture
title_full Direct communication of homologous and heterologous endocrine islet cells in culture
title_fullStr Direct communication of homologous and heterologous endocrine islet cells in culture
title_full_unstemmed Direct communication of homologous and heterologous endocrine islet cells in culture
title_short Direct communication of homologous and heterologous endocrine islet cells in culture
title_sort direct communication of homologous and heterologous endocrine islet cells in culture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112014/
https://www.ncbi.nlm.nih.gov/pubmed/7035468