A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells

We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double- staining experiments the lo...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112016/
https://www.ncbi.nlm.nih.gov/pubmed/6120174
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collection PubMed
description We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double- staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin.
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spelling pubmed-21120162008-05-01 A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells J Cell Biol Articles We have obtained a hybridoma clone, JLJ5a, which secretes monospecific antibody directed against a 110-kdalton protein of gerbil fibroma cells, Rat-1 fibroblasts, and L6 myoblasts. It appears to be localized in the Golgi apparatus by the following criteria: (a) In double- staining experiments the localization of the 110-kdalton protein by the JLJ5a monoclonal antibody was coincident with the reaction products of thiamine pyrophosphatase (one of the enzyme markers of the Golgi apparatus; Novikoff and Goldfischer, 1961, Proc. Natl. Acad. Sci. U.S.A. 47:802-810) in the same cells. (b) The staining pattern of the JLJ5a monoclonal antibody became fragmented and dispersed into vacuoles after pretreatment of the cells with Colcemid or monensin. The Rockefeller University Press 1982-01-01 /pmc/articles/PMC2112016/ /pubmed/6120174 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title_full A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title_fullStr A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title_full_unstemmed A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title_short A monoclonal antibody that recognizes Golgi-associated protein of cultured fibroblast cells
title_sort monoclonal antibody that recognizes golgi-associated protein of cultured fibroblast cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112016/
https://www.ncbi.nlm.nih.gov/pubmed/6120174

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