Cargando…
Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1982
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112108/ https://www.ncbi.nlm.nih.gov/pubmed/7068757 |
_version_ | 1782139880607842304 |
---|---|
collection | PubMed |
description | Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding. |
format | Text |
id | pubmed-2112108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1982 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21121082008-05-01 Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction J Cell Biol Articles Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding. The Rockefeller University Press 1982-04-01 /pmc/articles/PMC2112108/ /pubmed/7068757 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title | Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title_full | Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title_fullStr | Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title_full_unstemmed | Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title_short | Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
title_sort | release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112108/ https://www.ncbi.nlm.nih.gov/pubmed/7068757 |