Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction

Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112108/
https://www.ncbi.nlm.nih.gov/pubmed/7068757
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description Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.
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spelling pubmed-21121082008-05-01 Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction J Cell Biol Articles Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding. The Rockefeller University Press 1982-04-01 /pmc/articles/PMC2112108/ /pubmed/7068757 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title_full Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title_fullStr Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title_full_unstemmed Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title_short Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
title_sort release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112108/
https://www.ncbi.nlm.nih.gov/pubmed/7068757

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