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Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum
A rapid and simple method for the isolation of membranes from subcellular organelles is described. The procedure consists of diluting the organelles in ice-cold 100 mM Na2CO3 followed by centrifugation to pellet the membranes. Closed vesicles are converted to open membrane sheets, and content protei...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1982
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112113/ https://www.ncbi.nlm.nih.gov/pubmed/7068762 |