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Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells

Neural retinal cells from 9-d-old chick embryos were assayed for uridine diphosphate (UDP)-galactose:ganglioside GM2 galactosyltransferase, or GM1 synthetase, activity using the oligosaccharide fragment of GM2, oligo-GM2, oligo-GM2, as the exogenous acceptor. The results demonstrated that this enzym...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112116/
https://www.ncbi.nlm.nih.gov/pubmed/6802857
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collection PubMed
description Neural retinal cells from 9-d-old chick embryos were assayed for uridine diphosphate (UDP)-galactose:ganglioside GM2 galactosyltransferase, or GM1 synthetase, activity using the oligosaccharide fragment of GM2, oligo-GM2, oligo-GM2, as the exogenous acceptor. The results demonstrated that this enzyme activity was present on the external surfaces of intact cells. Little difference between the specific activities of cell surface GM1 synthetase could be detected when cells derived from dorsal and ventral segments of the neural retina were compared. These results suggested that this cell- surface enzyme was not present in a concentration gradient along the dorsoventral axis of the neural retina.
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spelling pubmed-21121162008-05-01 Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells J Cell Biol Articles Neural retinal cells from 9-d-old chick embryos were assayed for uridine diphosphate (UDP)-galactose:ganglioside GM2 galactosyltransferase, or GM1 synthetase, activity using the oligosaccharide fragment of GM2, oligo-GM2, oligo-GM2, as the exogenous acceptor. The results demonstrated that this enzyme activity was present on the external surfaces of intact cells. Little difference between the specific activities of cell surface GM1 synthetase could be detected when cells derived from dorsal and ventral segments of the neural retina were compared. These results suggested that this cell- surface enzyme was not present in a concentration gradient along the dorsoventral axis of the neural retina. The Rockefeller University Press 1982-04-01 /pmc/articles/PMC2112116/ /pubmed/6802857 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title_full Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title_fullStr Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title_full_unstemmed Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title_short Quantification of ganglioside GM1 synthetase activity on intact chick neural retinal cells
title_sort quantification of ganglioside gm1 synthetase activity on intact chick neural retinal cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112116/
https://www.ncbi.nlm.nih.gov/pubmed/6802857