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A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores
Phase contrast cine results demonstrate that erythrophores maintain saltatory particle motion for hours after permeabilization with 0.001% digitonin in a cytoskeletal stabilizing solution at 23 degrees C. High voltage electron microscopy (HVEM) studies reveal that cytoskeletal elements are retained...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1982
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112209/ https://www.ncbi.nlm.nih.gov/pubmed/6215414 |
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collection | PubMed |
description | Phase contrast cine results demonstrate that erythrophores maintain saltatory particle motion for hours after permeabilization with 0.001% digitonin in a cytoskeletal stabilizing solution at 23 degrees C. High voltage electron microscopy (HVEM) studies reveal that cytoskeletal elements are retained intact, except in immediate subplasmalemmal regions where the plasma membrane is punctured by digitonin. During digitonin treatments, cells are permeable to ions, small molecules, and antibodies. We find that motion is Ca2+ and ATP-sensitive, and optimal in PIPES buffer (pH 7.2 containing 1 mM Mg2+/ATP and EGTA-CA2+ (10(-7) M Ca2+) at 37 degrees C. Experiments testing the inhibitory effects of vanadate (0.4-10 microM), ouabain (100-600 microM), N-ethyl maleimide, and the cytochalasins B and D indicate that a dyneinlike ATPase may provide the motive force for driving saltatory pigment motion in erythropores. |
format | Text |
id | pubmed-2112209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1982 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21122092008-05-01 A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores J Cell Biol Articles Phase contrast cine results demonstrate that erythrophores maintain saltatory particle motion for hours after permeabilization with 0.001% digitonin in a cytoskeletal stabilizing solution at 23 degrees C. High voltage electron microscopy (HVEM) studies reveal that cytoskeletal elements are retained intact, except in immediate subplasmalemmal regions where the plasma membrane is punctured by digitonin. During digitonin treatments, cells are permeable to ions, small molecules, and antibodies. We find that motion is Ca2+ and ATP-sensitive, and optimal in PIPES buffer (pH 7.2 containing 1 mM Mg2+/ATP and EGTA-CA2+ (10(-7) M Ca2+) at 37 degrees C. Experiments testing the inhibitory effects of vanadate (0.4-10 microM), ouabain (100-600 microM), N-ethyl maleimide, and the cytochalasins B and D indicate that a dyneinlike ATPase may provide the motive force for driving saltatory pigment motion in erythropores. The Rockefeller University Press 1982-09-01 /pmc/articles/PMC2112209/ /pubmed/6215414 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title | A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title_full | A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title_fullStr | A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title_full_unstemmed | A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title_short | A functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
title_sort | functional in vitro model for studies of intracellular motility in digitonin-permeabilized erythrophores |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112209/ https://www.ncbi.nlm.nih.gov/pubmed/6215414 |