Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium

An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone- supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopress...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112226/
https://www.ncbi.nlm.nih.gov/pubmed/6182146
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collection PubMed
description An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone- supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopressin, and cholesterol. The hormone-defined medium (a) supports growth of LLC-PK1 cells at a rate of approaching that observed in serum-supplemented medium; (b) allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and (c) influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cyclic AMP. The medium has been used to isolate rabbit proximal tubular kidney epithelial cells free of fibroblasts.
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spelling pubmed-21122262008-05-01 Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium J Cell Biol Articles An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone- supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium, hydrocortisone, triiodothyronine, vasopressin, and cholesterol. The hormone-defined medium (a) supports growth of LLC-PK1 cells at a rate of approaching that observed in serum-supplemented medium; (b) allows vectorial transepithelial salt and fluid transport as measured by hemicyst formation; and (c) influences cell morphology. The vasopressin dependency for growth and morphology can be partially replaced by isobutylmethylxanthine or dibutyryl cyclic AMP. The medium has been used to isolate rabbit proximal tubular kidney epithelial cells free of fibroblasts. The Rockefeller University Press 1982-09-01 /pmc/articles/PMC2112226/ /pubmed/6182146 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title_full Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title_fullStr Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title_full_unstemmed Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title_short Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
title_sort continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112226/
https://www.ncbi.nlm.nih.gov/pubmed/6182146

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