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Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins
Rat liver plasma membranes were isolated as presented in the preceding paper (Hubbard, A. L., D. A. Wall, and A. Ma., 1983, J. Cell Biol., 96: 217-229) and found to contain many filaments associated both with desmosomes along the lateral surface and with the cytoplasmic aspects of membranes comprisi...
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Lenguaje: | English |
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The Rockefeller University Press
1983
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112253/ https://www.ncbi.nlm.nih.gov/pubmed/6681819 |
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collection | PubMed |
description | Rat liver plasma membranes were isolated as presented in the preceding paper (Hubbard, A. L., D. A. Wall, and A. Ma., 1983, J. Cell Biol., 96: 217-229) and found to contain many filaments associated both with desmosomes along the lateral surface and with the cytoplasmic aspects of membranes comprising each of the three domains (lateral [LS], bile canalicular [BC] and sinusoidal [SF] ). Exposure of the plasma membranes to alkaline media (up to pH 11) resulted in loss of recognizable filaments without loss of domain morphology or membrane enzyme activities. Electrophoretic analysis of solubilized components from control and alkaline-extracted plasma membranes revealed that three major polypeptides present at 43, 52, and 56 kdaltons in the control had been released by alkaline treatment (pH 11) and could be quantitatively recovered in the supernate. The 43-kdalton component was identified as cytoplasmic actin by comparison of its tryptic 125I- peptide map to those of muscle (alpha) and brush border (beta, gamma) actins. The 52- and 56-kdalton polypeptides were identified as tonofilament components by their solubility properties and their ability to reassemble into 9.5-nm filaments from monomers present in an alkaline extract. |
format | Text |
id | pubmed-2112253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21122532008-05-01 Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins J Cell Biol Articles Rat liver plasma membranes were isolated as presented in the preceding paper (Hubbard, A. L., D. A. Wall, and A. Ma., 1983, J. Cell Biol., 96: 217-229) and found to contain many filaments associated both with desmosomes along the lateral surface and with the cytoplasmic aspects of membranes comprising each of the three domains (lateral [LS], bile canalicular [BC] and sinusoidal [SF] ). Exposure of the plasma membranes to alkaline media (up to pH 11) resulted in loss of recognizable filaments without loss of domain morphology or membrane enzyme activities. Electrophoretic analysis of solubilized components from control and alkaline-extracted plasma membranes revealed that three major polypeptides present at 43, 52, and 56 kdaltons in the control had been released by alkaline treatment (pH 11) and could be quantitatively recovered in the supernate. The 43-kdalton component was identified as cytoplasmic actin by comparison of its tryptic 125I- peptide map to those of muscle (alpha) and brush border (beta, gamma) actins. The 52- and 56-kdalton polypeptides were identified as tonofilament components by their solubility properties and their ability to reassemble into 9.5-nm filaments from monomers present in an alkaline extract. The Rockefeller University Press 1983-01-01 /pmc/articles/PMC2112253/ /pubmed/6681819 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins |
title | Isolation of rat hepatocyte plasma membranes. II. Identification of
membrane-associated cytoskeletal proteins |
title_full | Isolation of rat hepatocyte plasma membranes. II. Identification of
membrane-associated cytoskeletal proteins |
title_fullStr | Isolation of rat hepatocyte plasma membranes. II. Identification of
membrane-associated cytoskeletal proteins |
title_full_unstemmed | Isolation of rat hepatocyte plasma membranes. II. Identification of
membrane-associated cytoskeletal proteins |
title_short | Isolation of rat hepatocyte plasma membranes. II. Identification of
membrane-associated cytoskeletal proteins |
title_sort | isolation of rat hepatocyte plasma membranes. ii. identification of
membrane-associated cytoskeletal proteins |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112253/ https://www.ncbi.nlm.nih.gov/pubmed/6681819 |