Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells

When L929 cells are exposed to 5 μg/ml dexamethasone, synthesis of a 90,000 M(r) polypeptide is induced within 12 h. Flattening of the cells begins at about this time and progresses to become quite prominent after 48 h of exposure. Two-dimensional PAGE and partial proteolytic fingerprints identify t...

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Detalles Bibliográficos
Autores principales: Lanks, KW, Kasambalides, EJ
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112297/
https://www.ncbi.nlm.nih.gov/pubmed/6300137
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author Lanks, KW
Kasambalides, EJ
author_facet Lanks, KW
Kasambalides, EJ
author_sort Lanks, KW
collection PubMed
description When L929 cells are exposed to 5 μg/ml dexamethasone, synthesis of a 90,000 M(r) polypeptide is induced within 12 h. Flattening of the cells begins at about this time and progresses to become quite prominent after 48 h of exposure. Two-dimensional PAGE and partial proteolytic fingerprints identify the 90,000 M(r) polypeptide as gelsolin, a Ca(++)-dependent inhibitor of actin polymerization. Thus, this system provides evidence that gelsolin may have a role in regulating cell shape in response to physiological agents such as glucocorticoids.
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spelling pubmed-21122972008-05-01 Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells Lanks, KW Kasambalides, EJ J Cell Biol Articles When L929 cells are exposed to 5 μg/ml dexamethasone, synthesis of a 90,000 M(r) polypeptide is induced within 12 h. Flattening of the cells begins at about this time and progresses to become quite prominent after 48 h of exposure. Two-dimensional PAGE and partial proteolytic fingerprints identify the 90,000 M(r) polypeptide as gelsolin, a Ca(++)-dependent inhibitor of actin polymerization. Thus, this system provides evidence that gelsolin may have a role in regulating cell shape in response to physiological agents such as glucocorticoids. The Rockefeller University Press 1983-02-01 /pmc/articles/PMC2112297/ /pubmed/6300137 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Lanks, KW
Kasambalides, EJ
Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title_full Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title_fullStr Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title_full_unstemmed Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title_short Dexamethasone induces gelsolin synthesis and altered morphology in L929 cells
title_sort dexamethasone induces gelsolin synthesis and altered morphology in l929 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112297/
https://www.ncbi.nlm.nih.gov/pubmed/6300137
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