Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes

Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the si...

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Detalles Bibliográficos
Autores principales: Kronenberg, HM, Fennick, BJ, Vasicek, TJ
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112328/
https://www.ncbi.nlm.nih.gov/pubmed/6300144
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author Kronenberg, HM
Fennick, BJ
Vasicek, TJ
author_facet Kronenberg, HM
Fennick, BJ
Vasicek, TJ
author_sort Kronenberg, HM
collection PubMed
description Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the signal sequence of β-lactamase, a secreted bacterial protein. We suggest that the apparent differences between bacterial and eukaryotic protein transport may involve only minor modulations of a profoundly conserved mechanism.
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spelling pubmed-21123282008-05-01 Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes Kronenberg, HM Fennick, BJ Vasicek, TJ J Cell Biol Articles Eucaryotic endoplasmic reticulum and bacterial inner cell membranes use strikingly similar mechanisms to transport proteins from one cellular compartment to another. Using a mammalian linked transcription-translation system, we show here that canine pancreatic microsomes accurately cleave off the signal sequence of β-lactamase, a secreted bacterial protein. We suggest that the apparent differences between bacterial and eukaryotic protein transport may involve only minor modulations of a profoundly conserved mechanism. The Rockefeller University Press 1983-04-01 /pmc/articles/PMC2112328/ /pubmed/6300144 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Kronenberg, HM
Fennick, BJ
Vasicek, TJ
Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title_full Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title_fullStr Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title_full_unstemmed Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title_short Transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
title_sort transport and cleavage of bacterial pre-β-lactamase by mammalian microsomes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112328/
https://www.ncbi.nlm.nih.gov/pubmed/6300144
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