Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster

The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded nati...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112364/
https://www.ncbi.nlm.nih.gov/pubmed/6815206
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description The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded native Drosophila salivary chromosomes. These chromosomes were then swollen and spread by a modified Miller procedure, stained or shadowed, and examined in the electron microscope. Individual nucleoprotein fibers were resolved with regularly repeated nucleosomes of approximately 10 nm diameter. Micrococcal nuclease digestion of isolated salivary nuclei gave a family of DNA fragments characteristic of nucleosomes for total chromatin, 5S gene, and simple satellite (rho = 1.688 g/cm3) sequences.
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spelling pubmed-21123642008-05-01 Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster J Cell Biol Articles The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded native Drosophila salivary chromosomes. These chromosomes were then swollen and spread by a modified Miller procedure, stained or shadowed, and examined in the electron microscope. Individual nucleoprotein fibers were resolved with regularly repeated nucleosomes of approximately 10 nm diameter. Micrococcal nuclease digestion of isolated salivary nuclei gave a family of DNA fragments characteristic of nucleosomes for total chromatin, 5S gene, and simple satellite (rho = 1.688 g/cm3) sequences. The Rockefeller University Press 1982-10-01 /pmc/articles/PMC2112364/ /pubmed/6815206 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title_full Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title_fullStr Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title_full_unstemmed Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title_short Nucleosome repeat structure is present in native salivary chromosomes of Drosophila melanogaster
title_sort nucleosome repeat structure is present in native salivary chromosomes of drosophila melanogaster
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112364/
https://www.ncbi.nlm.nih.gov/pubmed/6815206

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