The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein

A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. T...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112416/
https://www.ncbi.nlm.nih.gov/pubmed/6833379
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description A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A).
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spelling pubmed-21124162008-05-01 The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein J Cell Biol Articles A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A). The Rockefeller University Press 1983-03-01 /pmc/articles/PMC2112416/ /pubmed/6833379 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title_full The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title_fullStr The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title_full_unstemmed The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title_short The protein responsible for the repeating structure of cytoplasmic poly(A)-ribonucleoprotein
title_sort protein responsible for the repeating structure of cytoplasmic poly(a)-ribonucleoprotein
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112416/
https://www.ncbi.nlm.nih.gov/pubmed/6833379

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