Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions

Two mutants of Saccharomyces cerevisiae have been isolated from normal haploid MAT alpha strains and characterized as having temperature- sensitive, pleiotropic phenotypes for functions associated with mating. At the permissive temperature, 23 degrees C, they were found to behave as normal MAT alpha...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112447/
https://www.ncbi.nlm.nih.gov/pubmed/6343401
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collection PubMed
description Two mutants of Saccharomyces cerevisiae have been isolated from normal haploid MAT alpha strains and characterized as having temperature- sensitive, pleiotropic phenotypes for functions associated with mating. At the permissive temperature, 23 degrees C, they were found to behave as normal MAT alpha haploids with respect to mating efficiency, sporulation in diploids formed with MAT a strains, secretion of alpha- factor, and failure to secrete the MATa-specific products, a-factor and Barrier. At higher temperatures they were found to decline in mating and sporulation efficiency and to express the a-specific functions. Genetic analysis established that one of these mutants, PE34, carries a temperature-sensitive allele of the MAT alpha 2 gene and that the other, PD7, carries a temperature-sensitive allele of the TUP1 gene.
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spelling pubmed-21124472008-05-01 Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions J Cell Biol Articles Two mutants of Saccharomyces cerevisiae have been isolated from normal haploid MAT alpha strains and characterized as having temperature- sensitive, pleiotropic phenotypes for functions associated with mating. At the permissive temperature, 23 degrees C, they were found to behave as normal MAT alpha haploids with respect to mating efficiency, sporulation in diploids formed with MAT a strains, secretion of alpha- factor, and failure to secrete the MATa-specific products, a-factor and Barrier. At higher temperatures they were found to decline in mating and sporulation efficiency and to express the a-specific functions. Genetic analysis established that one of these mutants, PE34, carries a temperature-sensitive allele of the MAT alpha 2 gene and that the other, PD7, carries a temperature-sensitive allele of the TUP1 gene. The Rockefeller University Press 1983-06-01 /pmc/articles/PMC2112447/ /pubmed/6343401 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title_full Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title_fullStr Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title_full_unstemmed Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title_short Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions
title_sort two temperature-sensitive mutants of saccharomyces cerevisiae with altered expression of mating-type functions
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112447/
https://www.ncbi.nlm.nih.gov/pubmed/6343401

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