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Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus

Transformation by Rous sarcoma virus (RSV) has been reported to block the expression of differentiated cell products in chicken cells. The expression of these proteins may or may not be suppressed when temperature-sensitive mutants are shifted from the nonpermissive to the permissive temperature. A...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112459/
https://www.ncbi.nlm.nih.gov/pubmed/6304115
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description Transformation by Rous sarcoma virus (RSV) has been reported to block the expression of differentiated cell products in chicken cells. The expression of these proteins may or may not be suppressed when temperature-sensitive mutants are shifted from the nonpermissive to the permissive temperature. A general characteristic of cellular transformation is the disruption of the microfilament system. In passaged chick embryo fibroblast cultures (CEF), this system is principally composed of isomeric forms of actin designated alpha, beta, and gamma by their isoelectric focusing and when subjected to SDS-PAGE behavior. We present evidence that an alpha-actin in CEF cultures, identified by its electrofocusing behavior, retention in the cytoskeleton, and DNase 1 binding properties, is selectively and dramatically reduced in amount upon transformation by RSV. Little or no reduction is observed in the beta- and gamma-isoactins. The reduction of alpha-actin is shown to be reversible and transformation related by use of a temperature-sensitive mutant, tsNY68. The decrease in this transformation-sensitive isoactin is apparently due to a decrease in synthesis, though other possibilities are discussed. A specific decrease in a particular isoactin after transformation may give insight into the mechanism by which the microfilaments are normally maintained.
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spelling pubmed-21124592008-05-01 Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus J Cell Biol Articles Transformation by Rous sarcoma virus (RSV) has been reported to block the expression of differentiated cell products in chicken cells. The expression of these proteins may or may not be suppressed when temperature-sensitive mutants are shifted from the nonpermissive to the permissive temperature. A general characteristic of cellular transformation is the disruption of the microfilament system. In passaged chick embryo fibroblast cultures (CEF), this system is principally composed of isomeric forms of actin designated alpha, beta, and gamma by their isoelectric focusing and when subjected to SDS-PAGE behavior. We present evidence that an alpha-actin in CEF cultures, identified by its electrofocusing behavior, retention in the cytoskeleton, and DNase 1 binding properties, is selectively and dramatically reduced in amount upon transformation by RSV. Little or no reduction is observed in the beta- and gamma-isoactins. The reduction of alpha-actin is shown to be reversible and transformation related by use of a temperature-sensitive mutant, tsNY68. The decrease in this transformation-sensitive isoactin is apparently due to a decrease in synthesis, though other possibilities are discussed. A specific decrease in a particular isoactin after transformation may give insight into the mechanism by which the microfilaments are normally maintained. The Rockefeller University Press 1983-06-01 /pmc/articles/PMC2112459/ /pubmed/6304115 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title_full Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title_fullStr Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title_full_unstemmed Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title_short Transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by Rous sarcoma virus
title_sort transformation-sensitive isoactin in passaged chick embryo fibroblasts transformed by rous sarcoma virus
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112459/
https://www.ncbi.nlm.nih.gov/pubmed/6304115