Cargando…
Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane
The intracellular distribution of p39, a 39,000-dalton substrate for a number of tyrosine protein kinases, has been determined by indirect immunofluorescence microscopy. No binding of anti-p39 antibodies to intact cells was observed, indicating that this protein is not accessible to antibody on the...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1983
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112469/ https://www.ncbi.nlm.nih.gov/pubmed/6304112 |
_version_ | 1782139964706783232 |
---|---|
collection | PubMed |
description | The intracellular distribution of p39, a 39,000-dalton substrate for a number of tyrosine protein kinases, has been determined by indirect immunofluorescence microscopy. No binding of anti-p39 antibodies to intact cells was observed, indicating that this protein is not accessible to antibody on the cell surface. Following detergent permeabilization of formaldehyde-fixed cells, a reasonably uniform cytoplasmic labeling was observed. This fluorescence was most pronounced in membrane ruffles, especially in the leading lamellae of migrating cells, and in areas of cell-cell contact. Brief permeabilization of cells with detergent prior to formaldehyde fixation resulted in the appearance of a reticular lattice. An identical staining pattern was observed when fluorescently-labeled lectins were used as plasma membrane markers, but not when antibodies to a variety of cytoskeletal proteins were used. Taken together, these results indicate that p39 is, at least in part, located at the cytoplasmic surface of the plasma membrane. Immunolabeling of Rous sarcoma virus- transformed cells with anti-p39 antibodies resulted in fluorescent staining patterns indistinguishable from those observed in untransformed cells. It is conceivable that p39 plays some structural role within a protein network underlying the plasma membrane. |
format | Text |
id | pubmed-2112469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21124692008-05-01 Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane J Cell Biol Articles The intracellular distribution of p39, a 39,000-dalton substrate for a number of tyrosine protein kinases, has been determined by indirect immunofluorescence microscopy. No binding of anti-p39 antibodies to intact cells was observed, indicating that this protein is not accessible to antibody on the cell surface. Following detergent permeabilization of formaldehyde-fixed cells, a reasonably uniform cytoplasmic labeling was observed. This fluorescence was most pronounced in membrane ruffles, especially in the leading lamellae of migrating cells, and in areas of cell-cell contact. Brief permeabilization of cells with detergent prior to formaldehyde fixation resulted in the appearance of a reticular lattice. An identical staining pattern was observed when fluorescently-labeled lectins were used as plasma membrane markers, but not when antibodies to a variety of cytoskeletal proteins were used. Taken together, these results indicate that p39 is, at least in part, located at the cytoplasmic surface of the plasma membrane. Immunolabeling of Rous sarcoma virus- transformed cells with anti-p39 antibodies resulted in fluorescent staining patterns indistinguishable from those observed in untransformed cells. It is conceivable that p39 plays some structural role within a protein network underlying the plasma membrane. The Rockefeller University Press 1983-06-01 /pmc/articles/PMC2112469/ /pubmed/6304112 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title | Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title_full | Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title_fullStr | Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title_full_unstemmed | Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title_short | Immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
title_sort | immunofluorescent localization of a 39,000-dalton substrate of tyrosine protein kinases to the cytoplasmic surface of the plasma membrane |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112469/ https://www.ncbi.nlm.nih.gov/pubmed/6304112 |