A consistent picture of the actin filament related to the orientation of the actin molecule

We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and wi...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112490/
https://www.ncbi.nlm.nih.gov/pubmed/6190821
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collection PubMed
description We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament.
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spelling pubmed-21124902008-05-01 A consistent picture of the actin filament related to the orientation of the actin molecule J Cell Biol Articles We show that freeze-dried actin filaments which have been rotary shadowed with a light coat of platinum appear very similar in morphology and width to negatively-stained filaments. The addition of a thicker coat of platinum to such preparations gives the actin filaments a different morphology and width, which are similar to those of the rotary-shadowed, quick-frozen filaments described by Heuser and Kirschner (J. Cell Biol. 1980, 86:212-234). The consistent view of the actin filament presented here, particularly its 7-8-nm width, can be interpreted in terms of the overall orientation of the actin subunit in the actin filament. The Rockefeller University Press 1983-07-01 /pmc/articles/PMC2112490/ /pubmed/6190821 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A consistent picture of the actin filament related to the orientation of the actin molecule
title A consistent picture of the actin filament related to the orientation of the actin molecule
title_full A consistent picture of the actin filament related to the orientation of the actin molecule
title_fullStr A consistent picture of the actin filament related to the orientation of the actin molecule
title_full_unstemmed A consistent picture of the actin filament related to the orientation of the actin molecule
title_short A consistent picture of the actin filament related to the orientation of the actin molecule
title_sort consistent picture of the actin filament related to the orientation of the actin molecule
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112490/
https://www.ncbi.nlm.nih.gov/pubmed/6190821

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