Protein loss during nuclear isolation

Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112592/
https://www.ncbi.nlm.nih.gov/pubmed/6619193
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description Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.
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spelling pubmed-21125922008-05-01 Protein loss during nuclear isolation J Cell Biol Articles Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells. The Rockefeller University Press 1983-10-01 /pmc/articles/PMC2112592/ /pubmed/6619193 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Protein loss during nuclear isolation
title Protein loss during nuclear isolation
title_full Protein loss during nuclear isolation
title_fullStr Protein loss during nuclear isolation
title_full_unstemmed Protein loss during nuclear isolation
title_short Protein loss during nuclear isolation
title_sort protein loss during nuclear isolation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112592/
https://www.ncbi.nlm.nih.gov/pubmed/6619193

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