Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis

Friend murine erythroleukemia cells underwent apparently normal erythropoiesis when treated with dimethyl sulfoxide. One of the earliest events associated with this induction was a decrease in ouabain sensitive 86Rb+ uptake, an assay of the plasma membrane Na,K(ATPase). Ammonium vanadate (10 microM)...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1983
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112600/
https://www.ncbi.nlm.nih.gov/pubmed/6311842
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collection PubMed
description Friend murine erythroleukemia cells underwent apparently normal erythropoiesis when treated with dimethyl sulfoxide. One of the earliest events associated with this induction was a decrease in ouabain sensitive 86Rb+ uptake, an assay of the plasma membrane Na,K(ATPase). Ammonium vanadate (10 microM) blocked differentiation of these cells without affecting cell viability. Vanadium was taken up by Friend cells and prevented the dimethyl sulfoxide-induced decrease in ouabain sensitive 86Rb+ uptake. Vanadate reactivated 86Rb+ transport previously inhibited by dimethyl sulfoxide treatment but had no affect on 86Rb+ transport in untreated cells. These results suggest an essential role for the (Na,K)ATPase in cell differentiation.
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spelling pubmed-21126002008-05-01 Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis J Cell Biol Articles Friend murine erythroleukemia cells underwent apparently normal erythropoiesis when treated with dimethyl sulfoxide. One of the earliest events associated with this induction was a decrease in ouabain sensitive 86Rb+ uptake, an assay of the plasma membrane Na,K(ATPase). Ammonium vanadate (10 microM) blocked differentiation of these cells without affecting cell viability. Vanadium was taken up by Friend cells and prevented the dimethyl sulfoxide-induced decrease in ouabain sensitive 86Rb+ uptake. Vanadate reactivated 86Rb+ transport previously inhibited by dimethyl sulfoxide treatment but had no affect on 86Rb+ transport in untreated cells. These results suggest an essential role for the (Na,K)ATPase in cell differentiation. The Rockefeller University Press 1983-10-01 /pmc/articles/PMC2112600/ /pubmed/6311842 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title_full Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title_fullStr Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title_full_unstemmed Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title_short Vanadium stimulates the (Na+,K+) pump in friend erythroleukemia cells and blocks erythropoiesis
title_sort vanadium stimulates the (na+,k+) pump in friend erythroleukemia cells and blocks erythropoiesis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112600/
https://www.ncbi.nlm.nih.gov/pubmed/6311842

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