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Ion currents and membrane domains in the cleaving Xenopus egg
We used an extracellular vibrating probe to measure ion currents through the cleaving Xenopus laevis egg. Measurements indicate sharp membrane heterogeneities. Current leaves the first cleavage furrow after new, unpigmented membrane is inserted. This outward current may be carried by K+ efflux. No d...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1983
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112734/ https://www.ncbi.nlm.nih.gov/pubmed/6643577 |
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collection | PubMed |
description | We used an extracellular vibrating probe to measure ion currents through the cleaving Xenopus laevis egg. Measurements indicate sharp membrane heterogeneities. Current leaves the first cleavage furrow after new, unpigmented membrane is inserted. This outward current may be carried by K+ efflux. No direct involvement of the Na+,K+-ATPase in the generation of this outward current is detected at first cleavage. Inward current enters the old, pigmented membrane; however, it does not enter uniformly. The inward current is largest at the old membrane bordering the new membrane. This suggests a heterogeneous ion channel distribution within the old membrane. Experiments suggest that the inward current may be carried by Na+ influx, Ca2+ influx, and Cl- efflux. No steady currents were detected during grey crescent formation, the surface contraction waves preceding cleavage, or with groove formation at the beginning of cleavage. |
format | Text |
id | pubmed-2112734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1983 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21127342008-05-01 Ion currents and membrane domains in the cleaving Xenopus egg J Cell Biol Articles We used an extracellular vibrating probe to measure ion currents through the cleaving Xenopus laevis egg. Measurements indicate sharp membrane heterogeneities. Current leaves the first cleavage furrow after new, unpigmented membrane is inserted. This outward current may be carried by K+ efflux. No direct involvement of the Na+,K+-ATPase in the generation of this outward current is detected at first cleavage. Inward current enters the old, pigmented membrane; however, it does not enter uniformly. The inward current is largest at the old membrane bordering the new membrane. This suggests a heterogeneous ion channel distribution within the old membrane. Experiments suggest that the inward current may be carried by Na+ influx, Ca2+ influx, and Cl- efflux. No steady currents were detected during grey crescent formation, the surface contraction waves preceding cleavage, or with groove formation at the beginning of cleavage. The Rockefeller University Press 1983-12-01 /pmc/articles/PMC2112734/ /pubmed/6643577 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Ion currents and membrane domains in the cleaving Xenopus egg |
title | Ion currents and membrane domains in the cleaving Xenopus egg |
title_full | Ion currents and membrane domains in the cleaving Xenopus egg |
title_fullStr | Ion currents and membrane domains in the cleaving Xenopus egg |
title_full_unstemmed | Ion currents and membrane domains in the cleaving Xenopus egg |
title_short | Ion currents and membrane domains in the cleaving Xenopus egg |
title_sort | ion currents and membrane domains in the cleaving xenopus egg |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112734/ https://www.ncbi.nlm.nih.gov/pubmed/6643577 |