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Mechanism of action of cytochalasin: evidence that it binds to actin filament ends
To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to...
| Formato: | Texto |
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| Lenguaje: | English |
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The Rockefeller University Press
1981
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112756/ https://www.ncbi.nlm.nih.gov/pubmed/6894300 |
| _version_ | 1782140030817402880 |
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| collection | PubMed |
| description | To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to make filament ends (Brown and Spudich, 1979, J. Cell Biol. 80:499-504) and then reacted with [3H]cytochalasin B. We have found that cytochalasin B binds to beads in the presence of actin, and that the number of cytochalasin B binding sites can be varied as a function of the number of filament ends independent of the total actin concentration by varying the bead concentration. |
| format | Text |
| id | pubmed-2112756 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1981 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21127562008-05-01 Mechanism of action of cytochalasin: evidence that it binds to actin filament ends J Cell Biol Articles To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to make filament ends (Brown and Spudich, 1979, J. Cell Biol. 80:499-504) and then reacted with [3H]cytochalasin B. We have found that cytochalasin B binds to beads in the presence of actin, and that the number of cytochalasin B binding sites can be varied as a function of the number of filament ends independent of the total actin concentration by varying the bead concentration. The Rockefeller University Press 1981-03-01 /pmc/articles/PMC2112756/ /pubmed/6894300 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title | Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title_full | Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title_fullStr | Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title_full_unstemmed | Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title_short | Mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| title_sort | mechanism of action of cytochalasin: evidence that it binds to actin filament ends |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112756/ https://www.ncbi.nlm.nih.gov/pubmed/6894300 |