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Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes

Thin-section and critical-point-dried fracture-labeled preparations are used to determine the distribution and partition of glycophorin- associated wheat germ agglutinin (WGA) binding sites over protoplasmic and exoplasmic faces of freeze-fractured human erythrocyte membranes. Most wheat germ agglut...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1982
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112865/
https://www.ncbi.nlm.nih.gov/pubmed/7096449
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description Thin-section and critical-point-dried fracture-labeled preparations are used to determine the distribution and partition of glycophorin- associated wheat germ agglutinin (WGA) binding sites over protoplasmic and exoplasmic faces of freeze-fractured human erythrocyte membranes. Most wheat germ agglutinin binding sites are found over exoplasmic faces. Label is sparse over the protoplasmic faces. These results contrast with previous observations of the partition of band 3 component where biochemical analysis and fracture-label of concanavalin A (Con A) binding sites show preferential partition of this transmembrane protein with the protoplasmic face. Presence of characteristic proportions of WGA and Con A binding sites over each fracture face is interpreted to indicate the operation of a stochastic process during freeze-fracture. This process appears modulated by the relative expression of each transmembrane protein at either surface as well as by their association to components of the erythrocyte membrane skeleton.
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spelling pubmed-21128652008-05-01 Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes J Cell Biol Articles Thin-section and critical-point-dried fracture-labeled preparations are used to determine the distribution and partition of glycophorin- associated wheat germ agglutinin (WGA) binding sites over protoplasmic and exoplasmic faces of freeze-fractured human erythrocyte membranes. Most wheat germ agglutinin binding sites are found over exoplasmic faces. Label is sparse over the protoplasmic faces. These results contrast with previous observations of the partition of band 3 component where biochemical analysis and fracture-label of concanavalin A (Con A) binding sites show preferential partition of this transmembrane protein with the protoplasmic face. Presence of characteristic proportions of WGA and Con A binding sites over each fracture face is interpreted to indicate the operation of a stochastic process during freeze-fracture. This process appears modulated by the relative expression of each transmembrane protein at either surface as well as by their association to components of the erythrocyte membrane skeleton. The Rockefeller University Press 1982-05-01 /pmc/articles/PMC2112865/ /pubmed/7096449 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title_full Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title_fullStr Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title_full_unstemmed Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title_short Freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
title_sort freeze-fracture cytochemistry: partition of glycophorin in freeze- fractured human erythrocyte membranes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2112865/
https://www.ncbi.nlm.nih.gov/pubmed/7096449