Identification of coated vesicles in Saccharomyces cerevisiae

Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by r...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113006/
https://www.ncbi.nlm.nih.gov/pubmed/6368570
_version_ 1782140087273783296
collection PubMed
description Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by rotary shadowing. The contour length of a triskelion leg was 490 nm. Coated vesicle fractions contain a prominent band with molecular weight of approximately 185,000 when analyzed by SDS PAGE. The presence of coated vesicles in yeast cells suggests that this organism will be useful for studying the function of clathrin- coated vesicles.
format Text
id pubmed-2113006
institution National Center for Biotechnology Information
language English
publishDate 1984
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21130062008-05-01 Identification of coated vesicles in Saccharomyces cerevisiae J Cell Biol Articles Clathrin-coated vesicles were found in yeast, Saccharomyces cerevisiae, and enriched from spheroplasts by a rapid procedure utilizing gel filtration on Sephacryl S-1000. The coated vesicles (62-nm diam) were visualized by negative stain electron microscopy and clathrin triskelions were observed by rotary shadowing. The contour length of a triskelion leg was 490 nm. Coated vesicle fractions contain a prominent band with molecular weight of approximately 185,000 when analyzed by SDS PAGE. The presence of coated vesicles in yeast cells suggests that this organism will be useful for studying the function of clathrin- coated vesicles. The Rockefeller University Press 1984-01-01 /pmc/articles/PMC2113006/ /pubmed/6368570 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification of coated vesicles in Saccharomyces cerevisiae
title Identification of coated vesicles in Saccharomyces cerevisiae
title_full Identification of coated vesicles in Saccharomyces cerevisiae
title_fullStr Identification of coated vesicles in Saccharomyces cerevisiae
title_full_unstemmed Identification of coated vesicles in Saccharomyces cerevisiae
title_short Identification of coated vesicles in Saccharomyces cerevisiae
title_sort identification of coated vesicles in saccharomyces cerevisiae
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113006/
https://www.ncbi.nlm.nih.gov/pubmed/6368570

Ejemplares similares