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Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies

As the spike proteins of Semliki Forest virus (SFV) pass from their site of synthesis in the endoplasmic reticulum (ER) to the cell surface, they must be concentrated and freed from endogenous proteins. To determine the magnitude of this sorting process we have measured the density of spike proteins...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113041/
https://www.ncbi.nlm.nih.gov/pubmed/6563037
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description As the spike proteins of Semliki Forest virus (SFV) pass from their site of synthesis in the endoplasmic reticulum (ER) to the cell surface, they must be concentrated and freed from endogenous proteins. To determine the magnitude of this sorting process we have measured the density of spike proteins in membranes of the intracellular transport pathway. In this first paper, using stereological procedures, we have estimated the surface areas of the ER, Golgi complex, and plasma membrane of infected and mock-infected baby hamster kidney cells. First, we estimated the mean cell volume in absolute units. This was done using a novel in situ method which is described in detail. Infection by SFV was found to have no effect on any of the parameters measured. In the accompanying paper ( Quinn , P., G. Griffiths, and G. Warren, 1984, J. Cell Biol., 2142-2147) these stereological estimates were combined with biochemical estimates of the amount of spike proteins in ER, Golgi complex, and plasma membrane to determine the density in the membranes of these compartments.
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spelling pubmed-21130412008-05-01 Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies J Cell Biol Articles As the spike proteins of Semliki Forest virus (SFV) pass from their site of synthesis in the endoplasmic reticulum (ER) to the cell surface, they must be concentrated and freed from endogenous proteins. To determine the magnitude of this sorting process we have measured the density of spike proteins in membranes of the intracellular transport pathway. In this first paper, using stereological procedures, we have estimated the surface areas of the ER, Golgi complex, and plasma membrane of infected and mock-infected baby hamster kidney cells. First, we estimated the mean cell volume in absolute units. This was done using a novel in situ method which is described in detail. Infection by SFV was found to have no effect on any of the parameters measured. In the accompanying paper ( Quinn , P., G. Griffiths, and G. Warren, 1984, J. Cell Biol., 2142-2147) these stereological estimates were combined with biochemical estimates of the amount of spike proteins in ER, Golgi complex, and plasma membrane to determine the density in the membranes of these compartments. The Rockefeller University Press 1984-06-01 /pmc/articles/PMC2113041/ /pubmed/6563037 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title_full Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title_fullStr Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title_full_unstemmed Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title_short Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies
title_sort density of newly synthesized plasma membrane proteins in intracellular membranes. i. stereological studies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113041/
https://www.ncbi.nlm.nih.gov/pubmed/6563037