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Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells

When human erythroleukemic cells are induced to differentiate, they produce globin and redistribute glycophorin and spectrin to one pole of the cell. This process was accompanied by an alteration in the clathrin- coated pits at the cell surface. In nondifferentiating cells, receptors for Concanavali...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113073/
https://www.ncbi.nlm.nih.gov/pubmed/6144685
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description When human erythroleukemic cells are induced to differentiate, they produce globin and redistribute glycophorin and spectrin to one pole of the cell. This process was accompanied by an alteration in the clathrin- coated pits at the cell surface. In nondifferentiating cells, receptors for Concanavalin A have been shown, using electron microscopy, to be concentrated into coated pits and rapidly internalized. Glycophorin was also internalized via coated pits, but was not greatly concentrated into these portions of the surface membrane. Ligands attached to glycophorin were, therefore, cleared from the cell surface more slowly than Concanavalin A. In nondifferentiating cells, immunoelectron microscopy showed that spectrin is largely excluded from coated pits. After erythroid differentiation proceeded for several days, glycophorin was totally excluded from the coated pits along with spectrin. This did not reflect a general cessation of endocytosis, however, because Concanavalin A receptors continued to be internalized. It is possible that the specific exclusion of glycophorin from coated pits is part of the remodeling process that occurs when the precursor cell membrane differentiates into that of the mature erythrocyte.
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spelling pubmed-21130732008-05-01 Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells J Cell Biol Articles When human erythroleukemic cells are induced to differentiate, they produce globin and redistribute glycophorin and spectrin to one pole of the cell. This process was accompanied by an alteration in the clathrin- coated pits at the cell surface. In nondifferentiating cells, receptors for Concanavalin A have been shown, using electron microscopy, to be concentrated into coated pits and rapidly internalized. Glycophorin was also internalized via coated pits, but was not greatly concentrated into these portions of the surface membrane. Ligands attached to glycophorin were, therefore, cleared from the cell surface more slowly than Concanavalin A. In nondifferentiating cells, immunoelectron microscopy showed that spectrin is largely excluded from coated pits. After erythroid differentiation proceeded for several days, glycophorin was totally excluded from the coated pits along with spectrin. This did not reflect a general cessation of endocytosis, however, because Concanavalin A receptors continued to be internalized. It is possible that the specific exclusion of glycophorin from coated pits is part of the remodeling process that occurs when the precursor cell membrane differentiates into that of the mature erythrocyte. The Rockefeller University Press 1984-06-01 /pmc/articles/PMC2113073/ /pubmed/6144685 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title_full Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title_fullStr Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title_full_unstemmed Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title_short Exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
title_sort exclusion of erythrocyte-specific membrane proteins from clathrin- coated pits during differentiation of human erythroleukemic cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113073/
https://www.ncbi.nlm.nih.gov/pubmed/6144685