Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent

The effect of extracellular Ca2+ concentration and myasthenic globulin on the distribution and appearance of acetylcholine receptor (AChR) clusters on rat myotubes was studied with tetramethyl-rhodamine-labeled alpha BTX. Low Ca2+ medium (2.5 X 10(-5) M) caused a time-dependent loss of AChR clusters...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113093/
https://www.ncbi.nlm.nih.gov/pubmed/6693492
_version_ 1782140104757739520
collection PubMed
description The effect of extracellular Ca2+ concentration and myasthenic globulin on the distribution and appearance of acetylcholine receptor (AChR) clusters on rat myotubes was studied with tetramethyl-rhodamine-labeled alpha BTX. Low Ca2+ medium (2.5 X 10(-5) M) caused a time-dependent loss of AChR clusters, and a concomitant increase in small punctate areas of fluorescence. High Ca2+ concentrations (1.5 X 10(-2) M) increased the size of AChR clusters without altering AChR synthesis. These changes were not observed with other divalent ions. In the presence of myasthenic globulin, the rate of AChR turnover increases, and AChR clusters are rapidly dispersed. High Ca2+ concentration partially protects the AChR clusters from dispersal and decreases the rate of receptor turnover.
format Text
id pubmed-2113093
institution National Center for Biotechnology Information
language English
publishDate 1984
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21130932008-05-01 Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent J Cell Biol Articles The effect of extracellular Ca2+ concentration and myasthenic globulin on the distribution and appearance of acetylcholine receptor (AChR) clusters on rat myotubes was studied with tetramethyl-rhodamine-labeled alpha BTX. Low Ca2+ medium (2.5 X 10(-5) M) caused a time-dependent loss of AChR clusters, and a concomitant increase in small punctate areas of fluorescence. High Ca2+ concentrations (1.5 X 10(-2) M) increased the size of AChR clusters without altering AChR synthesis. These changes were not observed with other divalent ions. In the presence of myasthenic globulin, the rate of AChR turnover increases, and AChR clusters are rapidly dispersed. High Ca2+ concentration partially protects the AChR clusters from dispersal and decreases the rate of receptor turnover. The Rockefeller University Press 1984-02-01 /pmc/articles/PMC2113093/ /pubmed/6693492 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title_full Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title_fullStr Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title_full_unstemmed Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title_short Organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
title_sort organization of acetylcholine receptor clusters in cultured rat myotubes is calcium dependent
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113093/
https://www.ncbi.nlm.nih.gov/pubmed/6693492

Ejemplares similares