Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis

A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric transferrin. Analysis of the steps of the tra...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113163/
https://www.ncbi.nlm.nih.gov/pubmed/6321515
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collection PubMed
description A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric transferrin. Analysis of the steps of the transferrin cycle indicated that binding and internalization of transferrin proceeded normally in mutant cells. However, the mutant appeared unable to dissociate iron from transferrin, as evidenced by release of diferric transferrin from the mutant versus apotransferrin from the parent. Uptake of ferric ions from the growth medium was enhanced in the mutant.
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spelling pubmed-21131632008-05-01 Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis J Cell Biol Articles A Chinese hamster ovary cell mutant defective in the receptor-mediated endocytosis of several unrelated ligands (Robbins, A. R., S. S. Peng, and J. L. Marshall, 1983, J. Cell Biol., 96:1064-1071) failed to accumulate iron provided in the form of diferric transferrin. Analysis of the steps of the transferrin cycle indicated that binding and internalization of transferrin proceeded normally in mutant cells. However, the mutant appeared unable to dissociate iron from transferrin, as evidenced by release of diferric transferrin from the mutant versus apotransferrin from the parent. Uptake of ferric ions from the growth medium was enhanced in the mutant. The Rockefeller University Press 1984-03-01 /pmc/articles/PMC2113163/ /pubmed/6321515 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title_full Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title_fullStr Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title_full_unstemmed Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title_short Failure to release iron from transferrin in a Chinese hamster ovary cell mutant pleiotropically defective in endocytosis
title_sort failure to release iron from transferrin in a chinese hamster ovary cell mutant pleiotropically defective in endocytosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113163/
https://www.ncbi.nlm.nih.gov/pubmed/6321515

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