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Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts

The metabolism of the receptor for epidermal growth factor (EGF) has been measured by labeling the receptor in vivo with radioactive amino acid precursors and then determining, by immunoprecipitation with specific anti-EGF receptor antisera, the rate of degradation of the receptor when the cells are...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113166/
https://www.ncbi.nlm.nih.gov/pubmed/6321514
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description The metabolism of the receptor for epidermal growth factor (EGF) has been measured by labeling the receptor in vivo with radioactive amino acid precursors and then determining, by immunoprecipitation with specific anti-EGF receptor antisera, the rate of degradation of the receptor when the cells are placed in a nonradioactive medium. In human fibroblasts the rate of EGF receptor degradation (t1/2 = 10.1 h) was faster than the rate of degradation of total cell protein. When EGF was added to the nonradioactive medium, the half-life of prelabeled receptor was decreased to 1.2 h in human fibroblasts. These data demonstrate by direct analysis of receptor protein that during "down regulation" the EGF receptor is rapidly degraded. Enhanced receptor degradation was observed 5-10 min after the addition of EGF. The EGF- induced degradation of the receptor was blocked by methylamine, chloroquine, iodoacetate, or incubation at 25 degrees C. We have also shown that EGF-induced down regulation in human fibroblasts results in a decrease in the total amount of EGF receptor protein present. The amount of EGF receptor protein has been quantitated by radiolabeling cellular protein and immunoprecipitation of the receptor. The EGF receptor constitutes approximately 0.0035% of the cellular protein in human fibroblasts.
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spelling pubmed-21131662008-05-01 Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts J Cell Biol Articles The metabolism of the receptor for epidermal growth factor (EGF) has been measured by labeling the receptor in vivo with radioactive amino acid precursors and then determining, by immunoprecipitation with specific anti-EGF receptor antisera, the rate of degradation of the receptor when the cells are placed in a nonradioactive medium. In human fibroblasts the rate of EGF receptor degradation (t1/2 = 10.1 h) was faster than the rate of degradation of total cell protein. When EGF was added to the nonradioactive medium, the half-life of prelabeled receptor was decreased to 1.2 h in human fibroblasts. These data demonstrate by direct analysis of receptor protein that during "down regulation" the EGF receptor is rapidly degraded. Enhanced receptor degradation was observed 5-10 min after the addition of EGF. The EGF- induced degradation of the receptor was blocked by methylamine, chloroquine, iodoacetate, or incubation at 25 degrees C. We have also shown that EGF-induced down regulation in human fibroblasts results in a decrease in the total amount of EGF receptor protein present. The amount of EGF receptor protein has been quantitated by radiolabeling cellular protein and immunoprecipitation of the receptor. The EGF receptor constitutes approximately 0.0035% of the cellular protein in human fibroblasts. The Rockefeller University Press 1984-03-01 /pmc/articles/PMC2113166/ /pubmed/6321514 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title_full Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title_fullStr Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title_full_unstemmed Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title_short Down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
title_sort down regulation of epidermal growth factor receptors: direct demonstration of receptor degradation in human fibroblasts
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113166/
https://www.ncbi.nlm.nih.gov/pubmed/6321514