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Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982,...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1984
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113333/ https://www.ncbi.nlm.nih.gov/pubmed/6480696 |
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collection | PubMed |
description | We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982, Nature (Lond.), 295: 609-611; Wais-Steider, J., and P. Satir, 1979, J. Supramol. Struct., 11:339-347). Macrocilia contain several hundred axonemes linked into rows by lamellae between doublets 3 and 8. These connections provide morphological markers for numbering the doublet microtubules in thin sections. Demembranated, detached macrocilia undergo ATP-induced sliding disintegration by extrusion of thick fragments and finer fibers from the proximal end. Disintegration can easily be followed with low-magnification brightfield or phase-contrast optics. Sliding occurs with or without added elastase, and is reversibly inhibited by vanadate. Thin sections through 16 ATP-disintegrated macrocilia showed two mutually exclusive patterns of doublet extrusion with equal frequency. Doublets 9, 1, and 2 or doublets 5, 6, and 7 were usually extruded, but not both groups. We conclude that both subsets of doublets slide by their own active arms, and that the two extrusion patterns represent alternate activation and inactivation of doublet sliding on opposite halves of the axoneme. These findings provide the first direct experimental support for a switching mechanism regulating microtubule sliding in cilia. |
format | Text |
id | pubmed-2113333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1984 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21133332008-05-01 Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe J Cell Biol Articles We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982, Nature (Lond.), 295: 609-611; Wais-Steider, J., and P. Satir, 1979, J. Supramol. Struct., 11:339-347). Macrocilia contain several hundred axonemes linked into rows by lamellae between doublets 3 and 8. These connections provide morphological markers for numbering the doublet microtubules in thin sections. Demembranated, detached macrocilia undergo ATP-induced sliding disintegration by extrusion of thick fragments and finer fibers from the proximal end. Disintegration can easily be followed with low-magnification brightfield or phase-contrast optics. Sliding occurs with or without added elastase, and is reversibly inhibited by vanadate. Thin sections through 16 ATP-disintegrated macrocilia showed two mutually exclusive patterns of doublet extrusion with equal frequency. Doublets 9, 1, and 2 or doublets 5, 6, and 7 were usually extruded, but not both groups. We conclude that both subsets of doublets slide by their own active arms, and that the two extrusion patterns represent alternate activation and inactivation of doublet sliding on opposite halves of the axoneme. These findings provide the first direct experimental support for a switching mechanism regulating microtubule sliding in cilia. The Rockefeller University Press 1984-10-01 /pmc/articles/PMC2113333/ /pubmed/6480696 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title | Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title_full | Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title_fullStr | Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title_full_unstemmed | Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title_short | Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe |
title_sort | alternate patterns of doublet microtubule sliding in atp-disintegrated macrocilia of the ctenophore beroe |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113333/ https://www.ncbi.nlm.nih.gov/pubmed/6480696 |