Cargando…

Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe

We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982,...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1984
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113333/
https://www.ncbi.nlm.nih.gov/pubmed/6480696
_version_ 1782140161027473408
collection PubMed
description We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982, Nature (Lond.), 295: 609-611; Wais-Steider, J., and P. Satir, 1979, J. Supramol. Struct., 11:339-347). Macrocilia contain several hundred axonemes linked into rows by lamellae between doublets 3 and 8. These connections provide morphological markers for numbering the doublet microtubules in thin sections. Demembranated, detached macrocilia undergo ATP-induced sliding disintegration by extrusion of thick fragments and finer fibers from the proximal end. Disintegration can easily be followed with low-magnification brightfield or phase-contrast optics. Sliding occurs with or without added elastase, and is reversibly inhibited by vanadate. Thin sections through 16 ATP-disintegrated macrocilia showed two mutually exclusive patterns of doublet extrusion with equal frequency. Doublets 9, 1, and 2 or doublets 5, 6, and 7 were usually extruded, but not both groups. We conclude that both subsets of doublets slide by their own active arms, and that the two extrusion patterns represent alternate activation and inactivation of doublet sliding on opposite halves of the axoneme. These findings provide the first direct experimental support for a switching mechanism regulating microtubule sliding in cilia.
format Text
id pubmed-2113333
institution National Center for Biotechnology Information
language English
publishDate 1984
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21133332008-05-01 Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe J Cell Biol Articles We have used the unique properties of macrocilia from the lips of the ctenophore Beroe to test whether the ciliary beat cycle is caused by sequential activation of doublet sliding on opposite sides of the axoneme (Satir, P., 1982, Soc. Exp. Biol. Symp., 35: 179-201; Sugino, K., and Y. Naitoh, 1982, Nature (Lond.), 295: 609-611; Wais-Steider, J., and P. Satir, 1979, J. Supramol. Struct., 11:339-347). Macrocilia contain several hundred axonemes linked into rows by lamellae between doublets 3 and 8. These connections provide morphological markers for numbering the doublet microtubules in thin sections. Demembranated, detached macrocilia undergo ATP-induced sliding disintegration by extrusion of thick fragments and finer fibers from the proximal end. Disintegration can easily be followed with low-magnification brightfield or phase-contrast optics. Sliding occurs with or without added elastase, and is reversibly inhibited by vanadate. Thin sections through 16 ATP-disintegrated macrocilia showed two mutually exclusive patterns of doublet extrusion with equal frequency. Doublets 9, 1, and 2 or doublets 5, 6, and 7 were usually extruded, but not both groups. We conclude that both subsets of doublets slide by their own active arms, and that the two extrusion patterns represent alternate activation and inactivation of doublet sliding on opposite halves of the axoneme. These findings provide the first direct experimental support for a switching mechanism regulating microtubule sliding in cilia. The Rockefeller University Press 1984-10-01 /pmc/articles/PMC2113333/ /pubmed/6480696 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title_full Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title_fullStr Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title_full_unstemmed Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title_short Alternate patterns of doublet microtubule sliding in ATP-disintegrated macrocilia of the ctenophore Beroe
title_sort alternate patterns of doublet microtubule sliding in atp-disintegrated macrocilia of the ctenophore beroe
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2113333/
https://www.ncbi.nlm.nih.gov/pubmed/6480696